Double haploid techniques have been widely used to accelerate plant breeding， and in recent years， maize （Zea mays L.） lines containing mutations in the membrane protein （DMP） of the functionally unknown domain DUF679 have been used as haploidy-induced lines. In this study， soybean DMP genes with more than 60% homology with maize DMP gene were searched and bioinformatic analysis was performed. The molecular mechanism and biodiversity of GmDMP gene in soybean were investigated by using 2214 resequencing databases. The analysis results show that： GmDMP1 （Glyma.18G097400） and GmDMP2 （Glyma.18G098300） have high evolutionary affinity with maize DMP gene. The full length of genes is 645 bp， the homology rate of amino acid sequence is over 95%， and the number of encoded amino acids is completely consistent with the isoelectric point. The phosphorylation distribution differs at only one site. Both GmDMP1 and GmDMP2 genes have the same domain DUF679， which is more likely to locate in the ER， and both are transmembrane non-secreting hydrophilic proteins. There were 3 and 1 non-synonymous mutations of GmDMP1 and GmDMP2 genes in 2214 germplasm resources， respectively， consisting of 3 and 2 haplotypes. GmDMP1^H3 has been strongly selected during domestication， and the mutation site of the GmDMP1^H1 and GmDMP1^H2 haplotypes is located in the DUF679 domain. It is possible to obtain a haploid inducible line by mutating two DMP genes in soybean， thus shortening the breeding life of soybean.