Analysis of the Rice Blast Resistance Gene(s) in a Guangdong Landrace Xianluozhan
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    Abstract:

    The deployment of broad-spectrum resistance genes is the most effective and economic approach for controlling rice blast. The landrace Xianluozhan from South China displayed a broad-spectrum resistance against rice blast fungus. The resistant spectrum of the near isogenic line NIL-XLZ, derived from the Xianluozhan with the blast susceptible cultivar Lijiangxintuanheigu as a recurrent parent, is higher than that of the near isogenic lines of Pi2 and Piz and similar to that of Pi9 and Pi50. To identify the rice blast resistance gene(s) of Xianluozhan,we constructed an F2 population derived from Guanghui 290 (as a female parent) and Xianluozhan (as a male parent) for genetic analysis. The blast isolate GD08-T19 which had a broad virulence to rice cultivars from South China was used to inoculate the F1 and 1760 F2 individuals derived from the cross between Guanghui 290 and Xianluozhan. The genetic assay indicated that Xianluozhan carried a dominant blast resistance gene, since all of the F1 plants showed resistant and the segregation of resistant and susceptible progenies tend to fit a 3R:1S ratio in the F2 population. Based on the bulked segregant analysis (BSA) and recessive class analysis (RCA) with 44 microsatellite markers distributed around the loci of Pi2、Pi1 and Pita , the resistance gene hand been mapped in a 247 kb region of Pi2/Pi9/Pi50 family on chromosome 6 . The results of resistance spectrum analysis, gene specific markers detection and gene sequence comparison showed that Xianluozhan carried a broad spectrum blast resistance gene Pi50. This study provides useful information for the application of Xianluozhan in rice breeding.

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History
  • Received:January 28,2016
  • Revised:February 28,2016
  • Adopted:April 21,2016
  • Online: November 08,2016
  • Published:
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