For further study genetics diversity and relation of Chinese wolfberry germplasm, main morphological characters ,31 landrace wolfberry samples originally from China, 1 sample from USA and 1sample from Republic of Korea conserved in Ningxia were analyzed by Principal component and cluster methods in this paper. Results showed that these wolfberry materials had bigger differences for morphological characters, higher diversity for genetics. Evolution trends of morphological characters that leaf shape, wattle firmness and color, fruit color and flower organ, especially, fruit color from black →red →yellow, and leaf shape from lanceolate →strip lanceolate→strip evolution trends were obviously. Genetics distance was longer between Ningxia yellow-fruit- wolfberry and Zhongning black fruit wolfberry, compared with genetics distances between Ningxia yellow-fruit- wolfberry and Ningxia wolfberry cultivars that Ningqi 1 or Ningqi 2. It confirmed again that Ningxia yellow-fruit wolfberry was a mutation of Ningxia wolfberry. Among genetic characters, wolfberry plant height, crown, ground diameter, natural plant type, foliage shape, wattle firmness, leaf position, fruit color and flower form played dominant roles. Therefore, they could be reference breeding indices for new cultivar selection. Chinese wolfberry germplasm could be clustered into 10 genetic groups. It was similar to botanical classification result that 7 species and 3 mutations. Genetic distances of between groups, as well as between 33 wolfberry materials, have been identified.