The self-incompatibility allele of 11 apple cultivars were tested in this article．Self-incompatibility apple primers were designed according to the conserved sequence of amino acid“FTQQYQ”and“anti－1/WⅠPNV”． In this article, 9 new self-incompatibility genes named S34-, S35-, S36-, S37-, S38-, S39-, S40-, S41-, S42-Allele respectively were isolated with enzyme digestion and DNA sequencing of aim fragment method, they were deposited in GeneBank under the accession numbers of EU310474, EU391605, EU391606, EU391607, EU391609, EU391610, EU391611, EU391612 and EU391613. The self-incompatibility Alleles of 11 apple cultivars were: Sansa(S40S40), Fangming(S1S9), Yanga1(S38S27),Hongjingala(S39S27),Yanga2(S38S27),Qing9(S41S42),Asi(S36S36),Huangjiagala(S37S27), Jingxiang(S1S9S34),Gaoling(S38S9), Hongao(S35S35).