Forty radish germplasm were inoculated with strain Xcc8004 and XccBJ of Xanthomonas campestris pv. campestris (Xcc) at seedling stage. The fleshy root of eight germplasm among them were sliced and inoculated with strain Xcc8004. Twenty-seven accessions of them were inoculated with eight effectors. The result indicated that radish germplasm’s resistance to black rot was significantly different. Three accessions showed highly resistant (HR), one resistant (R), and four moderately resistant (MR) to Xcc8004. One HR, two R, and five MR radish germplasms to XccBJ were identified. The resistance of radish seedling to Xcc8004 was significantly correlated to the resistance to XccBJ, and the resistance of seedlings and fleshy roots to Xcc8004 was also significantly correlated. Seventeen radish germplasms exhibited hypersensitive response to different effectors. The number of radish germplasms showing hypersensitive response to XC_0241 was the most, followed by hypersensitive response to XC_0542 and XC_0541. The extent of hypersensitive response in different germplasm to different effectors was not identical. The reliably resistant resources provide the basic materials in breeding for the radish resistance and studying resistance mechanism.