YAV-2/TEZ//A.SQ(895) is a synthetic hexaploid wheat derived from a cross between T. durum Desf. and T. tauschii L., which is immune to powdery mildew. Genetic analysis of resistance to powdery mildew in F2 population and the F3 derived lines of YAV-2/TEZ//A.SQ(895)/3/Pinzi 50098 indicated a single dominant gene controlled the resistance. By bulk segregation analysis, microsatellite marker Xcfa2086 was identified to be closely linked to the resistance gene with genetic distance of 11.8cM. Based on the location of the linked microsatellite markers, the resistance gene was located on the telomeric region of chromosome 2AL in synthetic hexaploid wheat.