1.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences/State Key Laboratory of Crop Gene Resources and Breeding/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization,Ministry of Agriculture and Rural Affairs;2.Lishui University;3.Chongqing Academy of Agricultural Sciences;4.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/State Key Laboratory of Crop Gene Resources and Breeding/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization, Ministry of Agriculture and Rural Affairs
Strategic Cooperation Project on Agricultural Science and Technology Innovation between Chongqing Municipal People's Government and the Chinese Academy of Agricultural Sciences; National Key Research and Development Program of China (2021YFD1200500); The Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences;PublicWelfare Technology Application Research Program of Lishui City (2022GYX21)
Rice storage tolerance is a key trait for rice production and seed preservation, which is directly associated with food security and industrial economic benefits. Establishing a standardized identification system for rice storage tolerance is of great significance to the screening of storage-tolerant germplasm, breeding utilization and high-quality development of the rice industry. Current research on rice storage tolerance faces two core bottlenecks. First, there are no unified criteria for seed aging identification parameters, making it difficult to conduct horizontal comparison of experimental results obtained by different research institutions. Second, the comprehensive evaluation index system remains imperfect and lacks operable standardized grading criteria, which severely restricts the screening storage-tolerant rice germplasm and the progress of rice breeding. In view of the above problems, based on years of identification practices and research achievements, the paper systematically summarizes the technical methods and operational procedures for storage tolerance identification. A set of technical specifications covering sample preparation, instruments and reagents, identification procedures, as well as grading and judgment rules has been formulated, and unified and standardized technical operation criteria have been established. This study aims to provide standardized technical support for the identification of storage tolerance in rice germplasm resources and relevant breeding work. This specification is applicable to the identification and evaluation of seed storage tolerance of Oryza sativa L. seeds, and has important practical significance for standardizing the technical system of rice storage tolerance identification, implementing the national food security strategy and improving industrial economic benefits.
