Sweet potato (Ipomoea batatas (L.) Lam.) is a globally important crop for food, feed, and industrial raw materials. The long-term safe preservation of its germplasm resources is of great strategic significance for breeding innovation, industrial development and food security. Cryopreservation provides an important approach for the safe, economical, and long-term preservation of vegetatively propagated germplasm resources. However, the cryopreservation of sweet potato is generally hindered by bottlenecks such as difficulty in survival and regrowth. In this study, using the sweet potato cultivar 'Mianshu ' as the materials, i.e. ‘Mianshu 12’, , we optimized the droplet-vitrification cryopreservation protocol, focusing on exploring the effects of PVS2 treatment duration, compositions of vitrification solutions with varying DMSO concentrations. light quality composition during recovery culture, and plant growth regulator combinations in the culture medium on the survival and regrowth of sweet potato shoot tips. Additionally, physiological response mechanisms were elucidated using differential scanning calorimetry (DSC) and chlorophyll content analysis. The results showed that the optimal droplet-vitrification cryopreservation procedure consisted of a PVS2 treatment duration of 30 min, which could enable the shoot tip cells to achieve stable glass state; the chlorophyll content decreased significantly during cryoprevation proceduresthe treatments before post-recovery; while the applicationuse of red-blue composite light during the recovery stage could significantly promote the regrowth of cryopreserved shoot tips, with the optimal ratio of red to blue light of 8:2; and the suitable recovery medium composition was MS + 0.5 mg/L GA? + 0.5 mg/L 6-BA, the highest survival rate reached 58.00% ± 6.35%, with a regrowth rate reached 30%±10%. Applying this optimized protocol on two accessions. This study will reveal the synergistic regrowth-promoting effects of red-blue composite light and plant growth regulator ratios combined with GA3and6-BA during post-cryopreservation recovery, providing both theoretical basis and practical solutions for overcoming technical bottlenecks in sweet potato germplasm cryopreservation.