Glucosinolates (GSLs) are a type of important secondary metabolites in cruciferous plants. GSLs and their degradation products are known with important roles in plant flavor, insect resistance and anti-cancer. Sulforaphane, the degradation product of glucoraphanin (GRA), is one of the most important anti-cancer substances. The BrAOP2 gene is a key factor that modulates the GRA content. To analyze if the GSLs content between the Chinese Cabbage-Cabbage Translocation Line A-4 was different with that of their parent Chinese Cabbage 85-1, 84 selfing progenies plants of A-4 and 85-1 were subjected to measure by High Performance Liquid Chromatography the GSLs content in rosette leaves and heading leaves at rosette, early heading and harvest head stage. The genomic and transcriptional diversity of the BrAOP2 gene were analyzed. The results showed that the average contents of total GSLs, aliphatic GSLs, PRO, GBN, GRA, NAP and GBC of A-4 selfing progenies plants were always higher than those of 85-1 in heading leaves and rosette leaves at all stages. Especially, the highest value on contents were observed in heading leaves at early heading stages. Except the content of NEO, the contents of total GSLs and other GSLs components in heading leaves were higher than those in rosette leaves. The lower expression and SNP/Indels of the BrAOP2 gene in A-4 selfing progenies plants if compared to 85-1 were observed. With these results, it is speculated that the increase on GRA content in A-4 selfing progenies plants associates with the expression of BrAOP2. Collectively, these results provided insights for further analyzing the GRA content in 85-1 and Chinese cabbage-cabbage translocation lines.