In order to evaluate the practicability of molecular markers linked to stalk rot resistance genes in maize, 159 maize inbred lines were used to screen resistance on stalk rot in fields, and eleven molecular markers linked to four resistance QTLs, qRfg1, qRfg2, RpiQI319-1 and RpiQI319-2 were tested. The results showed that more than half of the tested materials belong to middle or high resistance. The average of incidence rate of total materials is 26.30%, and the materials of incidence less than 30% accounted for 67.92%. The proportion of resistant germplasms in Huanghuaihai region is lower than that in Northeast region or Southwest region. Practicability of molecular markers was evaluated for the conformity between genotype and phenotype. Four molecular markers, STS01 (qRfg1), STSZ479 (qRfg2), bnlg1866 (RpiQI319-1) and bnlg1716 (RpiQI319-2) have the high conformity, 76.79%, 78.95%, 91.67%, and 73.33%, respectively. The average incidence rate of positive materials detected by above markers was 22.06%, 19.01%, 10.65%, and 19.63%, respectively. Thus，STS01, STSZ479, bnlg1866 and bnlg1716 can be used as effective markers to molecular assisted selection on stalk rot resistance in maize breeding.