Improving the efficiency of nitrogen absorption in maize has important significance. In consideration of the characteristics of transportation of NO3- in CLC protein family, this study clones the CLC family genes ZmCLCa of maize by homology cloning method. Based on bioinformatics analysis, we find that the protein has a voltage-gated chlorine ion channel structure domain and subcellular localization result shows that the protein is located on the cell membrane. Under 200 mmol/L KNO3 processing conditions, the content of NO3- in transgenic arabidopsis of strain is obviously higher than that in wild arabidopsis. Therefore, ZmCLCa gene likely plays an important role in the process of nitrogen absorption of maize..