During last decades, the wheat sharp eyespot disease has occurred in many winter production regions. To mine the accessions with good resistance to sharp eyespot, 102 synthetic hexaploid wheat lines from CIMMYT were evaluated for resistance to sharp eyespot in field and greenhouse at Jiangsu Province and Beijing from 2007 to 2010. The results showed that these synthetic wheat lines displayed different degree of the resistance, suggesting that the selection of sharp eyespot resistance source among these lines was effective. Compared with the common wheat cultivars Yangmai 158 and Yangmai 12, most of 102 synthetic wheat showed better resistance with lower disease index. Seven synthetic wheat lines consistently displayed better resistance to sharp eyespot at the four tested sites from 2007 to 2010. These 7 resistant synthetic wheat lines included ZC93、ZC111、ZC112、ZC123、ZC172、ZC206 and ZC221. Thus, these 7 synthetic wheat lines could be used as novel resistance sources in wheat breeding with resistance to sharp eyespot disease.