Cloning and Expression Analysis of AmDREB2C in Ammopiptanthus mongolicus

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National Natural Science Foundation of China

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    Ammopiptanthus mongolicus, the only evergreen broad-leaf shrub in the northwest desert of China, shows very strong resistance to cold and drought stresses. The complete coding region cDNA and genomic DNA fragments of the transcription factor AmDREB2C were cloned by PCR approach from A. mongolicus. Both the fragments contain 1191 base pairs, therefore AmDREB2C is intronless. The predicted AmDREB2C protein is composed of 396 amino acid residues, with a conserved AP2 domain and a nuclear localization signal. Expression analysis revealed that the transcription of AmDREB2C was induced by cold and drought stresses. Moreover, the cDNA fragment for AmDREB2C was successfully constructed into plant expression vector, laying a foundation for further functional analysis of this gene.

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  • Received:June 30,2014
  • Revised:October 21,2014
  • Adopted:February 03,2015
  • Online: February 03,2015
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