Southern corn rust (SCR) is an destructive disease in the summer maize area and causes more than 10% yield loss in disease epidemic years. Few SCR-resistance lines are known and the deficiency in inbred lines with different SCR-resistance genes is evident in breeding. For finding and utilizing the new SCR-resistance germplasm screening of 1589 accessions to SCR was carried out by inoculation in the fields during 2008-2012 in Nanning, Guangxi. The results showed that 26 accessions were highly resistant, accounting for 1.64%; 138 accessions were resistant, accounting for 8.62%; 382 were moderately resistant, for 24.04%; 489 were susceptible, accounting for 30.77%; 555 were highly susceptible, for 34.93%. There were more resistant type lines (HR, R and MR) in imported maize germplasm. By re-screening 26 accessions, including 18 inbred lines, 3 landraces，4 Zimbabwe lines and Dr11 from CIMMYT were confirmed with highly resistance to southern corn rust that will provide new SCR-resistance source for breeding in China.