Guizhou province is an important distribution area of genetic resources. Sandu Autonomous County located in the southeast of Qiannan Buyi and Miao autonomous prefecture with significant geographic, climate and ethic. The investigation, collection and arrangement of agricultural biological resources related to work and life of the Shui, Miao, Buyei and Yao national minoritiesminority were carried on out in 15 villages distributed in 5 townships．The survey collected 213 accessions of agricultural agri-biological resources，which included 73 accessions of crops, 54 accessions of vegetables and annual economic crops, 34 accessions of perennial fruits and 54 accessions of medicinal plants. A total of 59 accessions of elite accessions were screen out. At same time, the status, growth and decline of local agricultural biological resources, investigation and collection of resource types and utilization value were analyzed, and the utilization, protection and development of agricultural biological resources in Sandu county were discussed. In this paper，the current situation of local agricultural biological resources，the reasons for the growth and decline，the types and thevalue use of collected resources were analyzed． The protection and the development of agricultural biological resources in Sandu autonomous county were discussed．