Male sterility played important roles in carrot breeding. In this study, a wild petaloid male-sterile carrot (named: ‘Wuye-BY’) was obtained from the wild carrot in Hongshan district of Wuhan city, China. The wild petaloid male-sterile carrot was not obvious fleshy taproots. Its tender stems, leaf blades and petioles were dark green without pubescence, petal and anther. The number of calyxes is 8-10 and the number of filaments is 0-2. The chromosome number of the male sterility is 2n=18. The top of mericarps and filaments contained a large amount of nectars. The male sterility can freely receive the pollen of cultivated carrots. The hybrid F1 had obvious advantages in root size, root weight, carotene, total sugar and vitamin C content.