白杨无性系秋季水分参数与冬季抗寒性指标关系研究
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科技部“十一五”科技支撑计划(2006BAD03A0106)


The Study on Relationship between Water Parameters in Autumn and Cold Resistances in Winter of White Poplar Hybrid Clones
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    摘要:

    选用7个白杨杂种无性系1年生苗为材料,采用P-V技术于秋季落叶前测定各无性系枝叶水分参数Ψ0π、Ψ100π、RWC0、ROWC0、εmax,并于冬季不同时期测定了各无性系相对电导率、钾离子渗漏率、SOD酶活性和POD酶活性的动态变化,对无性系抗寒性进行了综合评定。无性系各项生理指标存在一定变化模式,相对电导率、钾离子渗漏率在12月份降至最低,以后逐步提高,无性系间在2月份变异最大;SOD酶活性和POD酶活性在各时期无性系间差异均较大;无性系抗寒能力评价结果表明,从落叶开始,抗寒能力逐渐提高,12月至翌年1月达到最大,2月份又迅速下降,此时无性系间抗寒能力差异也达到最大。将水分参数与抗寒性指标进行相关分析表明,大部分水分参数与冬季不同时期的抗寒性指标存在不同程度的相关性,特别是与春季指标相关更加显著,其中Ψ0π是相关性最为显著的指标,Ψ100π相关不明显。

    Abstract:

    Seven oneyearold white poplar hybrid clones were selected as the experimental materials and the cold resistance characters had been comprehensively assessed according to the analysis of the water parameters (Ψ0π,Ψ100π,RWC0,ROWC0 and εmax)by P-V technology before leaf abscission in autumn and the dynamic changes of relative conductivity,potassium leakage rate,SOD and POD activities in winter.The results showed that there was a certain changing pattern existed in the physiological indexes of the clones.Relative conductivity and potassium leakage rate had decreased to the minimum in December,then gradually increased and the largest variation in clones was in February.The activities of SOD and POD had significant difference in different periods among clones.Therefore,the cold resistances gradually increased from the beginning of defoliation and achieved its maximum in December and January of the next year and then decreased rapidly in February.The difference of cold resistances among the clones was the maximum in February.The data revealed that the most of water parameters and the cold resistances index had different correlation degree in different periods of winter.The correlation was most evident in spring,in which the Ψ0π was a most significantlyed index while Ψ100π was not .

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王进茂,梁海永,杨敏生.白杨无性系秋季水分参数与冬季抗寒性指标关系研究[J].植物遗传资源学报,2010,11(1):28-33.

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