云南省热带作物科学研究所
云南省热带作物科技创新体系建设专项资金项目(RF2025);;云南省基础研究专项面上项目(202501AT070387);云南省现代农业橡胶产业技术体系建设育种与繁育研究室(2025KJTX-12-2)
Yunnan Institute of Tropical Crops
Special Fund Project of Yunnan Tropical Crop Science and Technology Innovation System Construction(RF2025); General Program of Yunnan Provincial Basic Research Special Project(202501AT070387); Yunnan Modern Agricultural Rubber Industry Technology System Construction Breeding and Breeding Research Laboratory(2025KJTX-12-2)
因我国橡胶树栽培品种选育长期依赖于少数魏克汉种质作为骨干亲本,存在遗传基础狭窄的问题,系统评价国外引进魏克汉种质对拓宽育种亲本具有重要价值。本研究以82份国外引进橡胶树魏克汉种质种质为材料,对其茎围、干胶产量、干胶含量及死皮等级进行测定,分析性状变异特征、遗传多样性及相关性,并结合主成分分析与系统聚类开展综合评价。结果表明,参试种质茎围各年生长量随林龄增长持续上升,而变异系数随林龄增加呈波动下降趋势。干胶产量3年间均呈“低-升-降-升-缓慢下降”的季节变化规律,月变异系数变化范围为44.98%~122.70%,第1割年离散程度较高(最高达122.7%),第3割年变异系数程度降低(44.98%~49.63%);3年间干胶含量均值范围为34.27%~34.66%,变异系数为3.33%~6.27%,离散程度较低。2年死皮等级均值范围为2.04~2.64,变异系数为45.90%~53.90%,表现出较高的离散程度。遗传多样性指数(H'')13年茎围生长范围为1.61~1.87,3年干胶产量范围为1.41~1.97,2年死皮等级范围为1.75~2.01,群体遗传多样性均较为丰富。相关性分析表明,相邻年份间茎围呈极显著正相关(P<0.01),但相关性随林龄间隔增大而减弱,8a生后茎围与第2和第3割年干胶产量呈显著相关(P<0.05);干胶产量各割年间呈极显著正相关(P<0.01),早期产量可有效预测后期表现;干胶产量与干胶含量及死皮等级相关性不显著。主成分分析共提取6个特征值大于1的主成分,累计贡献率达88.45%,筛选得到9项核心评价指标。通过隶属函数综合筛选出8份兼具速生、高产、低死皮等特征的优异种质,分别来自斯里兰卡(53号Nab25、66号Nab20、65号RRIC36)、科特迪瓦(298号IRCA301、300号IRCA307)、马来西亚(173号RRIM501、135号RRIM703)和巴西(169号IAN873)。系统聚类将82份种质划分为6个类群,聚类结果与种质地理来源无显著相关性,其中类群Ⅳ和类群Ⅵ为集中了多数综合性状优异种质的优势类群。本研究明确了国外引进魏克汉种质农艺性状早期的表型遗传特征,筛选出的优异种质可作为亲本材料或直接进入育种试种环节加以重点利用。
Most of the Hevea brasiliensis cultivars have long adopted a limited number of Wickham germplasms as core parents in China, leading to a narrow genetic base. Consequently, systematic evaluation of foreign-introduced Wickham germplasms holds significant value for expanding the genetic diversity of breeding parents. In this study, 82 foreign-introduced H. brasiliensis Wickham germplasms were assessed for stem girth, dry rubber yield, dry rubber content, and tapping panel dryness (TPD) grade. The variation patterns, genetic diversity, and correlation of these traits were analyzed. Meanwhile, comprehensive evaluations were conducted using principal component analysis (PCA) and hierarchical cluster analysis. The results showed that the annual girth increment of tested germplasms increased continuously with stand age, while the coefficient of variation (CV) presented a fluctuating declining trend with increasing stand age..Dry rubber yield exhibited a seasonal variation pattern of “low–rise–fall–rise–slow decline” across the three years. The monthly CV for yield ranged from 44.98% to 122.70% with the highest dispersion observed in the first tapping year (peaking at 122.70% in July 2018) and relatively low variation in the third tapping year (44.98%–49.63% from August to December 2020). Mean dry rubber content remained stable between 34.27% and 34.66% over the three years, with CV values ranging from 3.33% to 6.27%, indicating low phenotypic dispersion. The mean tapping panel dryness (TPD) grade over the two-year period ranged from 2.04 to 2.64, with coefficients of variation (CV) of 45.90%–53.70%, indicating a high degree of phenotypic dispersion. The Shannon-Wiener diversity index (H'') revealed rich genetic diversity within the population, with values ranging from 1.61 to 1.87 for 13-year stem girth, 1.41 to 1.97 for dry rubber yield, and 1.75 to 2.01 for TPD grade. Correlation analysis showed that stem girth exhibited highly significant positive correlations (P < 0.01) between adjacent growth years, and such correlation gradually weakened as standage intervals increased.. The stem girth of 8-year-old H. brasiliensis showed significant positive correlations with dry rubber yield during the second and third tapping years (P?<?0.05). In addition, dry rubber yields across different tapping years presented highly significant positive correlations (P < 0.01), indicating that early yield performance can reliably predict the late-yielding capacity of H. brasiliensis germplasm. Notably, no significant correlations were observed between dry rubber yield and dry rubber content or TPD grade. PCA extracted six principal components with eigenvalues greater than 1, which collectively explained 88.45% of the total phenotypic variation and supported the screening of nine core evaluation indices.Through membership function analysis, eight excellent germplasms with fast-growing, high-yield and low-skinned characteristics were screened out, which were from Sri Lanka ( No.53 Nab25, No.66 Nab20, No.65 RRIC36 ), Côte d ''Ivoire ( No.298 IRCA301, No.300 IRCA307 ), Malaysia ( No.173 RRIM501, No.135 RRIM703 ) and Brazil ( No.169 IAN873 ).These superior resources were characterized by fast growth vigor, high rubber yield, and low TPD occurrence. Hierarchical cluster analysis partitioned the 82 resources into six discrete clusters. The clustering grouping exhibited no obvious correspondence to their geographic origins. Clusters IV and VI constituted the two largest groups, which contained most resources with outstanding comprehensive agronomic performance. This study clarified the early-phase phenotypic genetic characteristics of introduced Wickham germplasm and identifies superior resources that can be utilized as parental materials or advanced directly to breeding trials for prioritized exploitation.
