Lodging resistance in crops is a complex quantitative trait, and precise phenotyping is pivotal for mining lodging resistance genes and elucidating their underlying genetic mechanisms. However, accurate field-based evaluation of lodging resistance in rice germplasm resources has long been a major technical challenge for crop breeders. To address this issue, our research group has developed a standardized technical protocol building on prior relevant research. This protocol encompasses the entire workflow of field-based lodging resistance evaluation, specifying operational norms and basic requirements for all key stages: general experimental requirements, land preparation, planting practices, water management, fertilization regimes, pest and disease control, and lodging resistance assessment at the mature stage. It is intended to serve as a robust technical reference for the systematic evaluation of lodging resistance in rice germplasm resources. This protocol is applicable to the field-based evaluation of lodging resistance in mature rice germplasm resources across all rice-growing regions in China.