Sucrose synthase （SUS） plays a crucial role in plant growth and development， as well as in responses to abiotic stress and the formation of plant quality. To explore the effect of LbaSUS gene expression on soluble sugar accumulation in fruits， members of the LbaSUS gene family were identified using bioinformatic tools. The physicochemical properties， gene structure， conserved motifs， phylogeny and collinearity were systematically analyzed. The correlation between the expression of LbaSUS genes and soluble sugar content was further investigated via quantitative real-time PCR （qRT-PCR） combined with the dynamic changes in sucrose content during fruit development. Based on the prediction of physicochemical properties and subcellular localization， six LbaSUS genes were identified from the Lycium barbarum genome， encoding proteins ranging from 739 to 918 amino acids. These genes were unevenly distributed across five chromosomes. Phylogenetic analysis revealed that the LbaSUS gene family members can be divided into three subfamilies： SUS I， SUS II， and SUS III. Notably， the gene structures and conserved motif compositions of LbaSUS were highly conserved within the same subfamily. Additionally， the promoter regions of LbaSUS gene family members contain a large number of cis-acting elements associated with plant growth and development， hormone responses， and stress tolerance. qRT-PCR analysis indicated that the expression levels of LbaSUS1， LbaSUS5 and LbaSUS6 gradually decreased with fruit ripening； LbaSUS2 and LbaSUS3 exhibited an initial increase followed by a decline in expression， while LbaSUS4 expression showed no significant change during fruit development. Subcellular localization analysis indicated that LbaSUS3 protein is localized in the chloroplast. Correlation analysis demonstrated a significant positive correlation between LbaSUS3 expression level and sucrose content. Collectively， these findings provide a solid foundation for further functional analysis and characterization of SUS genes in Lycium barbarum.