1青海大学农林科学院,西宁810016;2农业农村部植物新品种测试西宁分中心,西宁810016
研究方向为分子生物学和植物新品种DUS测试,E-mail: chencuiyang@126.com
青海省农林科学院创新基金(2023-NKY-08)
1Academy of Agriculture and Forestry, Qinghai University, Xining 810016;2Xining Sub-Center of New Plant Varietiy Tests, Ministry of Agriculture and Rural Affairs, Xining 810016
Innovation Fund of Qinghai Academy of Agriculture and Forestry Sciences (2023-NKY-08)
为探索基于DUS测试性状的亚麻种质资源多样性分析,弥补NY/T 2562-2014《植物新品种特异性、一致性和稳定性测试指南 亚麻》在西北高原地区的适应性不足,以285份亚麻种质资源为材料,通过多样性分析、相关性分析、聚类分析及主成分分析等方法,探讨基于DUS测试性状的亚麻种质资源的遗传多样性分析与区域亚麻标准品种筛选。结果表明,285份亚麻种质资源的始花期、植株高度等14个数量性状变异系数范围为4.91%~29.55%,遗传多样性指数范围为1.021~2.073。花冠颜色、种皮颜色等7个质量性状和假质量性状的遗传多样性指数范围为0.225~0.991,表明所选亚麻品种的遗传多样性丰富。相关性分析表明,花不同部位的性状之间、株型与工艺长度等均存在显著的协同变异关系。聚类分析表明在欧式距离为20.13时,285份亚麻品种可以被分为6类,其中第Ⅰ类单株蒴果数高,第Ⅱ类花丝均为白色,第Ⅲ类处于中间水平,第Ⅳ类植株工艺长度较小,第Ⅴ类植株高度较高,第Ⅵ类千粒重较低。主成分分析提取到的7个主成分累积贡献率达到了74.502%,最终筛选出包含285份种质资源所有代码表达范围的20个代表性亚麻品种。本研究为亚麻种质资源鉴定方法提供新的思考,为亚麻DUS测试中标准品种的地域性选择提供指导。同时通过比较国际植物新品种保护联盟(UPOV,International Union for the Protection of New Varieties of Plants)的亚麻DUS测试指南,为我国亚麻DUS测试指南的修订提供参考。
To explore the diversity of flax germplasm resources based on DUS test traits and address the insufficient adaptability of NY/T 2562-2014 Guidelines for testing of distinctness, uniformity and stability of new plant varieties - flax in the Northwest Plateau region, this study employed diversity analysis, correlation analysis, cluster analysis and principal component analysis to investigate the genetic diversity of 285 flax germplasm resources based on DUS test traits and screen regional standard varieties. The results showed that the coefficient of variation for 14 quantitative traits (including initial flowering stage, plant height, etc.) of 285 flax germplasm accessions ranged from 4.91% to 29.55%, and the Shannon-Weaver diversity index varied from 1.021 to 2.073. The Shannon-Weaver diversity index of seven qualitative and pseudo-qualitative traits (such as corolla color and seed coat color, etc. ) ranged from 0.225 to 0.991, indicating rich genetic diversity among the tested flax varieties. Correlation analysis revealed significant synergistic variation relationships among traits of different floral parts, as well as between plant type and technical length. Cluster analysis classified the 285 flax varieties into six groups at a Euclidean distance of 20.13. Group Ⅰ had a high number of capsules per plant; Group Ⅱ featured uniformly white filaments; Group Ⅲ exhibited intermediate phenotypic levels; Group Ⅳ had shorter technical length; Group Ⅴ possessed taller plant height; Group Ⅵ showed lower 1000-kernel weight. Principal component analysis extracted seven principal components with a cumulative contribution rate of 74.502%. Finally, 20 representative flax varieties covering the full phenotypic code variation range of all 285 germplasm accessions were selected. This study provides a new perspective on the identification methods of flax germplasm resources and offers guidance for the regional selection of standard variety in flax DUS testing. In addition, by comparing with the UPOV DUS test guidelines for flax, this paper provides a reference for the revision of the national flax DUS test guidelines of China.
陈翠萍,刘洋.基于
