This study was conducted using an F?-generation recombinant inbred line (RIL) population consisting of 554 lines, developed from a cross between the edible cultivar “Jihuatian 1” (maternal parent) and the lowsucrose breeding line W191 (paternal parent). Multienvironment genetic dissection was performed to address the challenge of simultaneous improvement in both sucroserelated flavor and healthbeneficial fatty acid composition in peanut. The results demonstrated high heritability for all traits investigated, with broadsense heritability ranging from 0.76 to 0.94. Correlation analysis revealed strong negative correlations between sucrose content and saturated fatty acids—stearic acid, arachidic acid, and behenic acid (|r| = 0.84~0.93)—whereas weak positive correlations were observed between sucrose and unsaturated fatty acids, namely oleic acid and linoleic acid (r = 0.11~0.49).By combining bulked segregant analysis sequencing (BSAseq) with linkage mapping, twelve significant quantitative trait loci (QTLs) were identified on chromosomes A06 and A08. Among them, a major sucrose QTL,?qSUCA06?(LOD 27.43~66.72, PVE 52.26~66.99%), was physically localized to the 115.68~116.06?Mb interval on chromosome A06. Notably, this interval exhibited substantial overlap with major QTLs governing three saturated fatty acids—qSACA06?(stearic acid),?qAACA06?(arachidic acid), and?qBACA06?(behenic acid)—providing direct genetic evidence for the strong negative phenotypic correlation between sucrose and these saturated fatty acids. Additionally, chromosome A08 harbored a fattyacid QTL hotspot, in which the QTLs for oleic acid (qOACA08, LOD 6.85~32.97) and linoleic acid (qLACA08, LOD 7.48~22.06) were tightly linked but displayed opposite additive effects, consistent with the metabolic conversion catalyzed by the Δ12desaturase (FAD2) enzyme.In summary, this research identifies key genomic regions on chromosomes A06 and A08 that coordinately regulate sucrose and lipid metabolism in peanut kernels. The findings establish a theoretical foundation and offer concrete molecular targets for markerassisted breeding, paving the way for the development of superior edible peanut varieties that combine high sucrose content, high oleic acid, and low saturated fatty acids.