In order to systematically evaluate the salt-alkali tolerance of Leymus chinensis germplasm resources, this study used 70 L. chinensis germplasms as materials. Under the stress of 60 mmol/L mixed salt-alkali (pH=9.5), 18 salt-alkali tolerance-related indices were systematically determined, including germination rate, root length, plant height, biomass, and photosynthetic parameters. Their relative values and salt-alkali tolerance indices were also calculated. The salt-alkali tolerance was comprehensively evaluated using methods such as genetic diversity analysis, correlation analysis, principal component analysis, and cluster analysis. The results showed that the coefficient of variation (CV) of each trait ranged from 17% to 188%, indicating rich genetic variation potential. Correlation analysis revealed that significant correlations existed between multiple salt-alkali tolerance indices. Principal component analysis extracted 6 principal components with a cumulative contribution rate of 84.615%, and a comprehensive evaluation D-value was established to assess the salt-alkali tolerance of the resources. Cluster analysis classified the 70 germplasms into three types: salt-alkali tolerant type (15.71%), moderately salt-alkali tolerant type (18.57%), and sensitive type (65.72%). Furthermore, a regression model for salt-alkali tolerance evaluation (R2=0.968) based on 6 key indices was established, which exhibited a good prediction effect after verification. The excellent salt-alkali tolerant germplasms screened and the rapid evaluation model established in this study can provide a theoretical basis, material support, and methodological support for salt-alkali tolerance breeding of Leymus chinensis.