1.福建农林大学;2.中国农业科学院蔬菜花卉研究所;3.唐山师范学院;4.唐山师范学院生命科学系
国家重点研发计划课题(2023YFD2300901);呼和浩特市科技创新领域人才项目(2022RC-产研院-3);唐山师范学院校基金(科技)项目(20255129041)
1.Fujian Agriculture and Forestry University;2.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences;3.Tangshan Normal university
National Key Research and Development Program Projects(2023YFD2300901)); Hohhot Science and Technology Innovation Talent Projects(2022RC-Industry-Research Institute-3); Tangshan Normal University Scientific Research Fund Projects(20255129041)
蔷薇科(Rosaceae)植物作为重要的经济及观赏物种,其体细胞胚发生(SE)再生技术为植物品种改良与分子育种提供了高效的研究平台,也是研究细胞全能性及胚胎发育机制的重要体系。本文系统综述了蔷薇科植物SE的研究进展,指出基因型、外植体选择、植物生长调节剂的种类与配比、基本培养基成分,外源添加物以及培养条件是影响SE发生的关键因素。分子机制研究表明,SERK、WUS、AP2/ERF家族基因通过调控细胞重编程、激素信号及表观遗传动态参与体细胞胚发生。然而,蔷薇科植物SE仍面临基因型依赖性强、诱导效率低、再生体系不稳定等瓶颈。未来研究应结合多组学分析、基因编辑及表观遗传调控技术,进一步解析SE的分子网络,优化诱导策略,开发新型诱导剂,以突破遗传转化限制,推动蔷薇科植物分子设计育种与种质创新应用。
The Rosaceae family includes many important economic and ornamental species. Thesomatic embryogenesis (SE) regeneration technology provides an efficient platform for plant variety improvement and molecular breeding. It also serves as a valuable system for studying cellular totipotency and embryo development mechanisms. This review summarizes recent advancesin Rosaceae SE research. We summarize the key factors influencing SE induction, including genotype, explant type, plant growth regulator combinations, basal medium composition, exogenous additives, and culture conditions. Molecular studies show that SERK, WUS, and AP2/ERF family genes regulate SE by controlling cell reprogramming, hormone signaling, and epigenetic dynamics. Nevertheless, Rosaceae SE still faces majorchallenges, including strong genotype dependence, low induction efficiency, and unstable regeneration systems. Future perspectives highlight the integration of multi?omics analyses, gene?editing, and epigenetic regulation techniques. Researchers need to dissect the SE molecular network further, optimize induction strategies, and develop novel inducers. These advances will overcome genetic transformation limits and promote molecular design breeding and germplasm innovation in Rosaceae plants.
