1.中国农业科学院麻类研究所,长沙 410205;2.中国农业科学院棉花研究所,河南安阳 455000;3.国家盐碱地综合利用技术创新中心, 山东东营 257345;4.山东众力棉业科技有限公司,东营 257500;5.湖南农业大学农学院,长沙 410128
研究方向为作物种质资源学、生物信息学,E-mail: 18720339099@163.com
杨泽茂,研究方向为作物种质资源学、分子数量遗传学、生物信息学, E-mail: yangzemao@caas.cn
戴志刚,研究方向为作物种质资源学,E-mail: dzgmonkey@126.com
陈基权,研究方向为作物种质资源学,E-mail: chenjiquan@caas.cn
山东省重点研发计划(2024SFGC0402);国家自然科学基金(32472119)
1.Institute of Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205;2.Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang 455000,Henan;3.National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying 257345,Shandong;4.Shandong Zhongli Cotton Industry Technology Co., Ltd, Dongying 257500;5.College of Agronomy, Hunan Agricultural University, Changsha 410128
Foundation projects: Key R&D Program of Shandong Province, China(2024SFGC0402); National Natural Science Foundation of China(32472119)
黄麻是世界上种植面积最大的韧皮纤维作物,其耐盐性强,进一步筛选强耐盐碱黄麻种质对利用和改良我国丰富的盐碱地资源具有重要意义。为系统评价100份黄麻种质耐盐性,并筛选适宜盐碱地种植的优良材料,本研究通过室内水培试验,设置0、200、230和250 mmol/L NaCl处理,于苗期调查盐害指数,并利用混合线性模型分析基因型随机效应,获得各基因型耐盐性的最佳线性无偏预测值进行评分和分级。同时,在含盐量约4‰的盐碱大田中进行种植试验,于工艺成熟期测定株高和茎粗。结果表明,黄麻耐盐性广义遗传力约为0.299,属中等水平,鉴定出16份高耐盐种质;大田试验显示盐胁迫下黄麻生长性状具有丰富的遗传变异,其中茎粗与株高的变异系数分别为14.14%和12.50%,相关性分析发现茎粗与耐盐性呈显著正相关(r=0.267, P=0.008)。基于耐盐性、茎粗和株高进行聚类分析,黄麻种质分为9类,高耐盐-高产型11份、高耐盐-中产型13份、普通型16份、中产-中耐盐型12份、高产-盐敏感型16份、细茎-中耐盐型9份、矮秆-中耐盐型9份、矮秆-低耐盐型7份、盐敏感型5份,筛选出11份兼具耐盐与高产潜力的优良种质。本研究建立了室内苗期鉴定与大田成熟期性状分析相结合的黄麻耐盐性综合评价体系,为黄麻耐盐品种选育与盐碱地开发利用提供了优良种质资源和理论依据。
As the most widely cultivated bast fiber crop globally, jute (Corchorus spp.) exhibits remarkable salt tolerance characteristics. The screening of salt-alkali tolerant jute germplasms are crucial for the utilization and improvement of China′s abundant saline-alkali land resources. This study aimed to systematically evaluate the salt tolerance of 100 jute germplasms and identify superior materials suitable for cultivation in saline-alkali soils. Through indoor hydroponic experiments, with treatments of 0, 200, 230 and 250 mmol/L NaCl, the salt injury index was investigated during the seedling stage. A Linear Mixed Model (LMM) was employed to analyze genotypic random effects, for which the Best Linear Unbiased Prediction (BLUP) breeding values for salt tolerance were obtained for scoring and grading. Concurrently, field trials were established in natural saline-alkali soil with a salt content of approximately 4‰, where plant height and stem diameter were measured at the technological maturity stage. The results showed that the broad-sense heritability of salt tolerance in jute was approximately 0.299, indicating a moderate level of genetic control. Sixteen highly salt-tolerant accessions were identified. Field phenotypic analysis revealed abundant genetic variation in growth traits under salt stress , with coefficients of variation of 14.14% for stem diameter and 12.50% for plant height. Correlation analysis demonstrated a significant positive relationship between stem diameter and salt tolerance (r=0.267, P=0.008). Cluster analysis based on salt tolerance, stem diameter, and plant height categorized the germplasms into nine groups: high salt tolerance-high yielding type (11 accessions), high salt tolerance-medium yielding type (13 accessions), common type (16 accessions), medium yielding-medium salt tolerance type (12 accessions), high yielding-salt sensitive type (16 accessions), thin stem-medium salt tolerance type (9 accessions), dwarf-medium salt tolerance type (9 accessions), dwarf-low salt tolerance type (7 accessions), and salt sensitive type (5 accessions). This approach enabled the effective screening of 11 elite germplasm that combine high salt tolerance with high yield potential. This study establishes a comprehensive evaluation system for jute salt tolerance, integrating laboratory-based seedling screening and field-based trait analysis at maturity. It provides valuable germplasm resources and a theoretical foundation for breeding salt-tolerant jute varieties and utilizing saline-alkali lands.
徐心怡,邓灿辉,程超华,等.
