1.中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室,北京100081;2.青海大学农林科学院,西宁810016;3.云南省农业科学院 粮食作物研究所,昆明650205;4.贵州省农业科学院园艺研究所,贵阳550006;5.四川省农业科学院作物研究所,成都610066;6.青海大学省部共建三江源生态与高原农牧业国家重点实验室,西宁810016
研究方向为食用豆种质资源,E-mail: 1966254845@qq.com
刘 荣,研究方向为食用豆种质资源,E-mail: liurong@caas.cn
杨 涛,研究方向为食用豆种质资源,E-mail: yangtao02@caas.cn
宗绪晓,研究方向为食用豆种质资源,E-mail: zongxuxiao@caas.cn
国家重点研发计划项目(2021YFD1200105-02);国家自然科学基金(32272134); 国家现代农业产业技术体系-食用豆(CARS-08);中国农业科学院科技创新工程(01-ICS-07)
1.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/State Key Laboratory of Crop Gene Resources and Breeding, Beijing 100081;2.Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016;3.Institute of Grain Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205;4.Institute of Horticulture, Guizhou Academy of Agricultural Sciences, Guiyang 550006;5.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066;6.State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016
National Key Research and Development Program of China (2021YFD1200105-02); National Natural Science Foundation of China (32272134); China Agriculture Research System of MOF and MARA-Food Legumes (CARS-08); Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (01-ICS-07)
救荒野豌豆(Vicia sativa L.)是豆科野豌豆属一年生或二年生高蛋白冷季豆类,具有耐寒、耐旱、耐瘠薄的特性,被广泛用作绿肥和饲料,在作物轮作系统中发挥了重要作用。过去,我国对救荒野豌豆种质资源缺乏系统的调查收集和鉴定评价,为了进一步探索其利用价值,选育高产优异种质,本研究在贵州、青海、四川和云南地区采集了212份救荒野豌豆种质资源,进行田间试验,调查表型数据进行多样性分析和鉴定评价。结果表明,救荒野豌豆种质资源的16个农艺性状遗传多样性丰富,其中单株粒数变异系数最高,为70%;根据相关性分析,大部分性状之间呈极显著正相关;对来自不同地区的救荒野豌豆进行性状差异统计分析得出,贵州与四川地区的种质只在成熟期性状上差异极显著,青海、云南地区的种质与其他地区的种质在大部分性状上差异显著或极显著;主成分分析将16个农艺性状集中在累计贡献率达到81.1%的5个主成分,其中第1主成分主要与单株粒重、单株粒数、干荚长和单株荚数等产量性状相关,而第2主成分主要与粒长、百粒重和粒宽等籽粒大小相关;通过主成分三维聚类图分析发现,不同地区的种质在表型上存在明显分化,云南和青海的种质单独聚类成簇,而贵州和四川的种质聚在一起,说明川黔两地的资源在形态性状上较为相似。通过进一步的表型鉴定评价,筛选出两份救荒野豌豆多荚多粒种质,单株荚数均大于100个,单株粒数均大于800粒。同时还筛选到了2份大粒种质,其中1份具有早熟特征。以上结果为救荒野豌豆种质资源的保护利用和育种改良提供了宝贵资源和理论支撑。
Common vetch (Vicia sativa L.) is an annual or biennial legume with high protein content and strong adaptability under cold climates, drought conditions, and poor soil fertility. It is extensively utilized as a green manure and livestock feed, playing a crucial role in crop rotation systems. However, its systematic investigation, collection, identification and evaluation remain yet conducted to date in China. In order to explore its application potential and select high-yielding and superior germplasm varieties, this study collected 212 germplasm resources from the regions of Guizhou, Qinghai, Sichuan and Yunnan provinces, China. Field trials were conducted and phenotypic data was investigated for diversity analysis and identification. The results revealed a rich genetic diversity at 16 agronomic traits, with seed number per plant (SNPP, 70%) exhibiting the highest coefficient of variation. Correlation analysis indicated predominantly positive associations among most of the characters. Through statistical analysis between common vetch from different regions, maturity time traits was detected with extremely significant diversification between Guizhou and Sichuan, while variations at multiple trait were observed between Qinghai and Yunnan germplasm and other regions across multiple traits. Principal component analysis (PCA) condensed the 16 agronomic traits into five principal components, accounting for a cumulative contribution rate of 81.1%. The first principal component primarily encompassed yield-related traits such as grain weight per plant, grain number per plant, dry pod length, and pod number per plant, and the second principal component mainly represented grain size attributes including hundred-grain weight, grain length, and grain width. Three-dimensional cluster analysis based on PCA exhibited distinct phenotypic differentiation among germplasm from different regions. Germplasm from Yunnan and Qinghai formed separate clusters while those from Guizhou and Sichuan grouped together, indicating morphological trait similarities within Sichuan and Guizhou resources. Two elite germplasms were characterized by increasing number of pods and seeds, exhibiting the number of pods per plant exceeding 100, and SNPP surpassing 800. Two germplasms exhibiting large grain were identified, and one of them showed early maturity. These findings provide valuable resources and theoretical support for the conservation, utilization, and breeding enhancement of common vetch germplasm resources.
王宗赫,张红岩,杨新,等.救荒野豌豆种质资源调查收集及表型鉴定评价[J].植物遗传资源学报,2025,26(7):1318-1330.
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