利用猕猴桃雌雄花同株材料发掘性别决定相关基因
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1.福建农林大学园艺学院;2.中国科学院武汉植物园/中国科学院植物种质创新与特色农业重点实验室/中国科学院猕猴桃产业技术工程实验室;3.中国科学院大学

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中国科学院战略性先导科技专项(A类)(XDA24030404);国家现代农业产业技术体系(CARS-26)资助;陕西省重点研发计划(2023-ZDLNY-24);果树种质创新与利用湖北省重点实验室开放课题(GSSZ202301)


Sex Determination-Related Genes were Identified from the Monoecious Kiwifruit Flowers?
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1.College of Horticulture, Fujian Agriculture and Forestry University;2.Chinese Academy of Sciences Wuhan Botanical Garden/Chinese Academy of Sciences Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture/Chinese Academy of Sciences Engineering Laboratory for Kiwifruit Industrial Technology

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This work was supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences (Precision Seed Design and Breeding), (XDA24030404); Supported by the earmarked fund for CARS(CARS-26); Key R&D Project in Shaanxi Province (2023-ZDLNY-24); Supported by Hubei Key Laboratory of Germplasm Innovation and Utilization of Fruit Trees (Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences) (GSSZ202301)

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    摘要:

    为探究猕猴桃雌雄异株变异为雌雄同株的分子机制,本研究对满天红(‘MTH’)猕猴桃变异株的雌花与雄花进行了转录组测序及生物信息学分析。结果表明,雌花和雄花间共有 337 个差异表达基因,雌花中有 241 个基因表达量上调,而表达量下调的基因有 96 个。差异表达基因的 KEGG 富集结果表明,与雌花相比,雄花中上调的基因主要参与氨基糖和核苷酸糖的代谢以及次生代谢物合成等路径;下调的基因则重要富集在次生代谢物合成、类胡萝卜素合成等途径相关。通过功能注释分析出30个潜在的性别相关调控基因,其中5个在次生代谢产物合成路径中表达。选取7个差异表达基因进行qRT-PCR验证,其在雌雄花中的表达水平与转录组数据一致。本研究获得了‘MTH’猕猴桃变异株雌花与雄花的差异表达基因及功能注释信息,为猕猴桃雌雄同株材料的创制提供了一定的理论依据。

    Abstract:

    In order to explore the mechanism of domestication of kiwifruit dioecious plants into monoecious plants, transcriptome sequencing and bioinformatics analysis of female and male flowers of ' MTH ' kiwifruit mutants were carried out in this study. The results showed that there were 337 differentially expressed genes between female and male flowers, with 241 genes u-regulated and 96 genes down-regulated in female flowers. The KEGG enrichment results of differentially expressed genes showed that compared with female flowers, the up-regulated genes in male flowers were mainly involved in the metabolism of amino sugars and nucleotide sugars and the synthesis of secondary metabolites. The down-regulated genes were mainly enriched in secondary metabolite synthesis, carotenoid synthesis and other pathways. Through functional annotation analysis, 30 potential gender-related regulatory genes were identified, of which 5 were expressed in the secondary metabolite synthesis pathway. Moreover, seven differentially expressed genes were selected for qRT-PCR verification, and the results were consistent with the transcriptome data. In this study, the differentially expressed genes and functional annotation information of female and male flowers of ' MTH ' kiwifruit mutant were obtained, which provided a theoretical basis for monoecious kiwifruit breeding.

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  • 收稿日期:2023-12-03
  • 最后修改日期:2024-01-05
  • 录用日期:2024-01-25
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