内蒙古143份冰草属种质资源表型性状遗传多样性分析与综合评价
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作者单位:

1.中国农业科学院草原研究所;2.中国农业科学院作物科学研究所,甘肃农业大学草业学院;3.内蒙古大学;4.中国农业科学院作物科学研究所

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基金项目:

农作物种质资源保护与利用项目( 2023NWB037);内蒙古自治区科技重大专项(2021ZD0031);中央级公益性科研院所基本科研业务费专项(Y2023PT02)


Genetic diversity analysis and comprehensive evaluation of phenotypic traits of 143 Agropyron cristatum ( L.) Gaertn. germplasm resources in Inner Mongolia
Author:
Affiliation:

1.Institute of Grassland Research, CAAS;2.Crop Science Research Institute of CAAS,College of Prataculture, Gansu Agricultural University;3.Inner Mongolia University

Fund Project:

Crop Germplasm Resources Protection and Utilization Project (2023NWB037); Key Projects in Science and Technology of Inner Mongoli (2021ZD0031);The special item of basic scientific research business expenses of the Central Public Welfare Scientific Research Institute (Y2023PT02)

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    摘要:

    以来源于内蒙古10个盟市及地区的 143份冰草属种质资源为研究对象,通过变异系数、遗传多样性指数、相关性、主成分、聚类和灰色关联法等分析方法,分析了143份内蒙古地区冰草属种质资源表型性状的多样性水平,并筛选了优异种质资源材料,为冰草属种质资源创新和品种选育提供参考。结果表明:冰草属种质的17个表型性状变异较大,遗传多样性丰富,变异系数在93.92% ~ 11.47%之间,平均为42.80%,其中叶片宽度的变异系数最高,种子长的离散程度最低;遗传多样性指数(H")为 1.279 ~ 2.025,平均为1.72,以颖长度的多样性指数最大,小穗长的多样性指数最小;相关性分析表明,各性状间显著或极显著相关;主成分分析显示,前6个主成分因子贡献率为 5.934% ~ 20.885%,累计贡献率达 69.866%;聚类分析将143份种质划分为3大类。第Ⅰ类综合性状较好,小穗数和小花数指标突出,具有高产潜力,包含27份资源;第Ⅱ类综合性状一般,种子综合性状较高,包含23份资源;第Ⅲ类综合性状较差,株高较长,包含93份资源;采用灰色关联的方法综合评价冰草属种质的综合表现,千粒重、小穗数、叶舌长度、叶片宽度和叶面积作为种质评价指标;鉴选出A-24、A-23、A-25、A-22、A-35、A-43、A-18冰草材料及A-142、A-92和A-136 多花冰草材料等综合性状协调的优异种质,可供冰草属种质遗传改良与育种利用。

    Abstract:

    A total of 143 (Agropyron cristatum ( L.) Gaertn.) germplasm resources from 10 cities and regions in Inner Mongolia were studied.Through the analysis methods of coefficient of variation, genetic diversity index, correlation, principal component, clustering and grey correlation,The diversity level of phenotypic traits of 143 samples of Agropyron cristatum ( L.) Gaertn. germplasm resources in Inner Mongolia was analyzed, and excellent germplasm resources were screened, which provided reference for germplasm resource innovation and variety breeding of Agropyron cristatum ( L.) Gaertn.The results showed that the 17 phenotypic traits of the species had great variation and rich genetic diversity. The coefficient of variation ranged from 93.92% to 11.47%, with an average of 42.80%. The coefficient of variation of leaf width was the highest, and the dispersion of seed length was the lowest. The genetic diversity index (H") ranged from 1.279 to 2.025, with an average of 1.72. The diversity index of glume length was the highest, and lowest for spikelet length.Correlation analysis shows significant or extremely significant correlations between various traits.Principal component analysis showed that the contribution rate of the first six principal component factors was 5.934% ~ 20.885%, and the cumulative contribution rate was 69.866%.The 143 germplasm were divided into 3 groups by cluster analysis, and the characters of each group were different and the characteristics were prominent. Class I had the best comprehensive traits, with prominent spikelet number and floret number, and had high yield potential, including 27 resources. The second type, containing 23 resources, had general traits and higher seed traits. The third type was poor in general character and long in plant height, containing 93 resources. Agropyron cristatum ( L.) Gaertn. species have abundant phenotypic genetic diversity.It is feasible to use the grey correlation method to comprehensively evaluate the germplasm of Agropyron cristatum ( L.) Gaertn. The comprehensive performance of the species of Agropyron cristatum ( L.) Gaertn. could be quantified, and the 1000-grain weight, spikelet number, Leaf tongue length, leaf width and leaf area were used as germplasm evaluation indexes.Selected excellent germplasm with coordinated comprehensive traits such as A-24, A-23, A-25, A-22, A-35, A-43, A-18 A.cristatum materials, and A-142, A-92, and A-136 A.cristatum var.pluriflorum materials, which can be used for genetic improvement and breeding of ice grass germplasm.

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  • 收稿日期:2023-11-09
  • 最后修改日期:2024-01-08
  • 录用日期:2024-01-09
  • 在线发布日期: 2024-01-19
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