两个柠檬品种叶片离区响应乙烯利处理的转录组分析
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1.云南省农业科学院热带亚热带经济作物研究所;2.西南林业大学林学院

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国家自然科学基金项目(31960574);英才兴边计划(2022RC001);创新引导与科技型企业培育计划(202304BI090025)


Transcriptome analysis of leaf abscission zones post ethephon treatment in two lemon varieties
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The National Natural Science Foundation of China (31960574);Talent Revitalization Program(2022RC001); Innovation Leadership and Technology-based Business Incubation Program(202304BI090025)

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    摘要:

    ‘艾伦尤力克’是尤力克柠檬的一个芽变品种,结果性状优良,但冬季落叶严重,影响翌年产量,且其落叶的响应机制目前尚不清楚。为探究柠檬叶片脱落的分子调控机制,以2个冬季落叶程度不同的柠檬品种‘艾伦尤力克’和‘云柠1号’为材料,分别于落叶前期(E24)、落叶中期(E48)和落叶后期(E72)采集叶柄离区,通过转录组测序比较2个品种间的差异表达基因。分析表明,E24、E48和E72时期分别获得1400、2466和935个差异表达基因,其中E48时期的差异基因数量最多。GO分析表明血红素结合、四吡咯结合、氧化还原酶活性、铁离子结合、转录调节活性等相关基因在3个时期品种间均表现出显著差异。KEGG分析表明,E48时期富集的差异基因数及相关代谢途径最多,主要集中在植物激素信号转导、苯丙烷生物合成、植物病原体相互作用和MAPK信号通路-植物等途径,通过对4个通路的差异表达显著的基因进行分析,最后筛选得到木葡聚糖内转葡糖基酶/水解酶蛋白(CL4G051299012_alt、CL0G071451012_alt和CL4G051300012_alt)、吲哚乙酸诱导蛋白(CL9G066930012_alt、CL3G046634012_alt)、吲哚-3-乙酸-氨基合成酶 GH3(CL2G041491012_alt)、过氧化物酶(CL0G070710012_alt、CL2G041037012_alt、CL0G070975012_alt)、β-葡萄糖苷酶(CL7G062117012_alt、CL7G062118012_alt)、发病相关基因转录激活因子AP2(CL2G044356012_alt、CL8G064323012_alt)和发病机制相关蛋白(CL9G066888012_alt、CL8G064323012_alt)13个基因,这些基因可能与柠檬叶片脱落的调控有关。

    Abstract:

    'Allen Eureka' is a bud variety of Eureka lemon with excellent fruiting traits, but severe winter defoliation affects the following year's yield, and the response mechanism for its defoliation is currently unknown.Two lemon cultivars (‘Allen Eureka’ and ‘Yunning No. 1’) with various defoliation traits were used as materials to investigate the molecular regulatory mechanisms of leaf abscission in lemons. The petiole abscission zone was collected at three different defoliation stages, namely, the predefoliation stage (E24), the middefoliation stage (E48), and the postdefoliation stage (E72). Comparison of differentially expressed genes between two lemon varieties with different degrees of winter defoliation by transcriptome sequencing. A total of 1400, 2466, and 935 differentially expressed genes (DEGs) were obtained in CQ, CZ, and CH, respectively, and the number of DEGs in CZ was the largest. GO analysis revealed that the DEGs between the two cultivars were mainly enriched in processes related to heme binding, tetrapyrrole binding, oxidoreductase activity, iron ion binding and ranscription regulator activity in the defoliation stages. KEGG analysis showed that the DEGs were concentrated in the E48 and involved plant hormone signal transduction, phenylpropanoid biosynthesis, Plant-pathogen interaction, and MAPK signaling pathway - plant. By analyzing the genes with significant differential expression of the four pathways, the final screen yielded xyloglucan endotransglucosylase/hydrolase protein (CL4G051299012_alt, CL0G071451012_alt, and CL4G051300012_alt), Indoleacetic acid-induced protein (CL9G066930012_alt, CL3G046634012_alt),Indole-3-acetic acid-amido synthetase GH3 (CL2G041491012_alt), peroxidase (CL0G070710012_alt, CL2G041037012_alt, CL0G070975012_alt), β-glucosidase (CL7G062117012_alt, CL7G062118012_alt), Pathogenesis-related genes transcriptional activator AP2 (CL2G044356012_alt, CL8G064323012_alt) and Pathogenesis-related protein (CL9G066888012_alt, CL8G064323012 _alt) 15 genes that can be associated with the regulation of lemon leaf abscission.

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  • 收稿日期:2023-10-31
  • 最后修改日期:2024-01-10
  • 录用日期:2024-01-29
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