Abstract:Cadmium (Cd) stress severely limits plant growth, so it is particularly important to screen and identify genes related to cadmium tolerance in plants. In the early stage, we screened the transcriptome data to obtain tomato UDP-glycosyltransferase gene (SlUDP), which responds to cadmium stress in plants. In this study, we first cloned the full-length sequence of the coding region of SlUDP gene. Gene expression analysis showed that SlUDP was highly expressed in leaves and fruits, and its expression was up-regulated by cadmium stress. Yeast genetic transformation test showed that yeast strains containing INVSc1-pYES2-SlUDP showed certain tolerance under different concentrations of cadmium stress. SlUDP overexpression vector transformation model plant Arabidopsis Thaliana was constructed and overexpression Arabidopsis strain was obtained. When SlUDP overexpression occurs in Arabidopsis plants exposed to cadmium stress (40, 60, 80 μM) When used, its survival rate is>50%, while the survival rate of wild Arabidopsis is is<10%. At 60 μM CdCl2, compared with wild-type plants, the malondialdehyde content of SlUDP -overexpressed Arabidopsis plants decreased by about 1.4 times, and the soluble sugar content, superoxide dismutase activity and peroxidase activity increased by about 1.8, 2, 1.25 times. These results indicated that overexpression of SlUDP led to changes in the antioxidant enzyme system, increased the ability of plant to remove reactive oxygen species, reduced the degree of membrane lipid peroxidation, and gave plants better cadmium tolerance. In addition, the expression levels of metal ion transport-related genes (ZIP1, IRT1, COPT2 and CSD1) in overexpressed plants were 3.1, 1.5, 1.6, 2.1 times than those in wild type plants. These results suggest that tomato SlUDP gene may enhance plant tolerance to cadmium stress through active oxygen scavenging system and metal ion transport pathway. This study provides a theoretical basis for the study of the role of glycosyltransferase gene in plant tolerance to cadmium stress, and provides candidate genes for molecular breeding of horticultural plant resistance.