60份辣椒骨干亲本的SSR遗传多样性分析及指纹图谱构建
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江西省农业科学院 蔬菜花卉研究所

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江西省农作物良种联合攻关——辣椒优异种质创制及优质丰产新品种选育;国家现代农业产业技术体系专项资金项目(CARS-24-G-08);江西省现代农业产业技术体系建设专项(JXARS-06);江西现代农业科研协同创新专项——特色果菜、花卉种质资源收集评价与创新利用(JXXTCX202202)


Genetic Diversity and Fingerprint Analysis of 60 Pepper Founder Parents Based on SSR Markers
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Institute of Vegetable and Flowers,Jiangxi Academy of Agricultural Sciences

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Jiangxi Crop Improved Varieties Joint Project—Excellent germplasm creation of capsicum and breeding of new varieties with high quality and yield; China Agriculture Research System (CARS-24-G-08); Jiangxi Agriculture Research System (JXARS-6); Jiangxi Agricultural Research Collaborative Innovation Project—Collection, evaluation and innovative utilization of characteristic fruit vegetables and flower germplasm resources (JXXTCX202202)

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    摘要:

    为解析我省辣椒育种骨干亲本材料的遗传多样性并构建其DNA指纹图谱,本研究利用毛细管电泳和SSR分子标记技术对江西60份辣椒种质材料进行位点检测。结果表明,49对SSR引物共检测出202个等位基因,平均4.122个,有效等位基因平均2.172个,香农信息指数平均0.850,多态信息含量平均0.414,说明60份供试材料具有较为丰富的遗传多样性;期望杂合度平均0.475大于观测杂合度0.126,表明多代自交而导致材料纯合度较高。聚类分析、群体结构分析和主坐标分析结果一致,支持我省辣椒育种亲本材料以南方地区和小果型尖椒材料为主,遗传背景狭窄且保持较纯的血统。此外,本研究根据多位点匹配分析确定了PM1、CAMS-855、CO911525S、PM22、HpmsE015和Hpms1214为核心引物,并利用这6对核心引物构建了60份辣椒育种亲本的指纹图谱,为我省辣椒资源的鉴定和保护提供了有力支撑,为分子辅助育种中亲本的选择提供理论依据。

    Abstract:

    In order to analyze the genetic diversity of the founder parents of pepper breeding in Jiangxi Province and construct their DNA fingerprints, capillary electrophoresis and SSR molecular marker techniques were used to detect the loci of 60 pepper germplasm resources in Jiangxi Province. The results showed that a total of 202 alleles were detected by using 49 pairs of SSR primers, with an average of 4.122 alleles per pair of primers and an average of 2.172 effective alleles. The average Shannon’s information index was 0.850, and the average polymorphism information content (PIC) was 0.414, indicating that the tested materials had abundant genetic diversity. The average expected heterozygosity of 0.475 was greater than the observed heterozygosity of 0.126, indicating that the homozygosity of the tested materials was rather high due to continuous multi-generation selfing. The genetic distance clustering, population structure analysis and principal coordinate analysis (PCoA) were used to classify the tested materials, and 60 germplasms were divided into 3 groups, 2 subgroups and 3 sets, respectively. The results of the three classifications had good consistency, which supported that the parent materials of pepper breeding in Jiangxi province were mainly from the southern China region and small fruit type pepper materials, with narrow genetic background and relatively pure blood relationship. In addition, PM1, CAMS-855, CO911525S, PM22, HpmsE015 and Hpms1214 were identified as the core primers based on multi-locus matching analysis. Meanwhile, the DNA fingerprints of 60 pepper germplasms were constructed by using the selected 6 core primers. This study provides a reliable basis for the identification and protection of pepper resources, and for the selection of parents in molecular assisted breeding.

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  • 收稿日期:2023-08-30
  • 最后修改日期:2023-09-18
  • 录用日期:2023-09-19
  • 在线发布日期: 2024-06-28
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