大豆脯氨酸积累相关基因家族鉴定及干旱胁迫表达分析
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哈尔滨师范大学生命科学与技术学院 / 分子细胞遗传与遗传育种重点实验室

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国家重点研发计划项目(2021YFD1201103);国家自然科学基金(31972507);哈尔滨师范大学研究生创新基金(HSDSSCX2021-104)


Identification and Expression Analysis of Proline Accumulation Related Gene Families in Soybean under Drought Stress
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School of Life Science and Technology,Harbin Normal University/Key Laboratory of Molecular Cytogenetics and Genetic Breeding

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National Key Research and Development Program of China(2021YFD1201103):National Natural Science Foundation of China(31972507):Graduate Innovation Fund of Harbin Normal University(HSDSSCX2021-104)

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    摘要:

    脯氨酸是一种广泛存在的渗透调节物质,在植物生长发育以及响应干旱胁迫的信号途径中具有重要作用。吡咯啉-5-羧酸合成酶(P5CS)、鸟氨酸转氨酶(δ-OAT)、吡咯啉-5-羧酸还原酶(P5CR)、脯氨酸脱氢酶(ProDH)、吡咯啉-5-羧酸脱氢酶(P5CDH)、脯氨酸转运体(ProT)是影响植物体内脯氨酸积累的关键酶。但关于大豆脯氨酸积累相关基因家族成员的研究尚未见报道。本研究在大豆基因组中鉴定出7个GmP5CS、2个GmOAT、2个GmP5CR、5个GmProDH、3个GmP5CDH及6个GmProT基因,不均匀地分布在大豆20条染色体中的12条上,发生16对片段复制事件。系统进化树分析发现,大豆脯氨酸积累相关基因家族分为不同的进化分支,同一亚族间的基因结构和保守基序相似。顺式作用元件分析表明,脯氨酸积累相关基因家族含响应逆境胁迫及植物激素的顺式作用元件。干旱胁迫下的表达模式分析表明,脯氨酸合成代谢(GmP5CS、GmOAT、GmP5CR)相关基因家族成员响应干旱胁迫,在干旱胁迫24 h时显著上调表达;脯氨酸分解代谢相关基因家族成员(GmProDH、GmP5CDH)的表达量显著下调,脯氨酸转运相关基因家族成员(GmProT)在干旱胁迫24 h显著上调表达,其中GmP5CS5、GmOAT1、GmProT亚族II及GmProDH3~5基因在干旱胁迫下的脯氨酸积累中可能起到关键作用。大豆幼苗P5CS、OAT活性随干旱胁迫时间的延长呈显著上升的趋势,与脯氨酸的积累呈正相关:ProDH活性随干旱胁迫时间的增长呈显著下降的趋势,与脯氨酸的积累呈负相关。本研究为进一步解析大豆脯氨酸积累相关家族基因响应干旱胁迫的功能提供了参考。

    Abstract:

    Proline is a ubiquitous osmotic regulator that plays an important role in plant growth and development as well as signaling pathways in response to drought stress. P5CS, δ-OAT, P5CR, ProDH, P5CDH and ProT are the key enzymes that affect proline accumulation in plants. However, the members of the gene families related to proline accumulation in soybean remained to be systemically investigated. In this study, seven GmP5CS, two GmOAT, two GmP5CR, five GmProDH, three GmP5CDH and six GmProT genes were identified and found to be unevenly distributed on 12 of 20 soybean chromosomes, showing 16 pairs of fragment duplication events. The phylogenetic analysis showed that the soybean proline accumulation-related gene families were classified into different evolutionary branches, while within each branch a conservation on functional structure and motifs was observed. Analysis of cis-acting elements in these gene families revealed contained cis-acting elements that were reported associating with response to stress and plant hormones. Analysis of transcriptional profiles under drought stress treatment showed that the proline anabolism-related (GmP5CS, GmOAT, GmP5CR) gene family members were significantly up-regulated 24 hours post treatment, the proline catabolism-related gene family members (GmProDH, GmP5CDH) were significantly down-regulated, and the expression levels of proline transport-related gene family members (GmProT) were significantly up-regulated. Especially, GmP5CS5, GmOAT1, GmProT subgroup II and GmProDH3-5 genes may play key roles in proline accumulation under drought stress. Moreover, the P5CS and OAT activity of soybean seedlings, which was significantly increased along with the increase of drought stress time, positively correlated with proline accumulation. The ProDH activity was significantly decreased along with the increase of drought stress time, and was negatively correlated with the accumulation of proline. Collectively, this study provided information for further analyzing the functions of soybean proline accumulation-related family genes in response to drought stress.

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引用本文

商成慧,周泽宇,崔文雪,等.大豆脯氨酸积累相关基因家族鉴定及干旱胁迫表达分析[J].植物遗传资源学报,2022,23(6):1793-1806.

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  • 收稿日期:2022-03-28
  • 最后修改日期:2022-07-27
  • 录用日期:2022-07-27
  • 在线发布日期: 2022-11-16
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