利用高密度SNP芯片定位玉米雄穗分枝数QTL
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1.黑龙江大学现代农业与生态环境学院;2.北京市农林科学院玉米研究中心 / 玉米 DNA 指纹及分子育种北京市重点实验室

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“十三五”国家重点研发计划(2016YFD0101803)


QTL Mapping of the Tassel Branch Number Using High-density SNP Array in Maize
Author:
Affiliation:

1.College of Advanced Agriculture and Ecological Environment of Heilongjiang University;2.Maize Research Center,Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding

Fund Project:

National Key Research and Development Program of the 13th Five-year Plan(2016YFD0101803)

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    摘要:

    玉米雄穗分枝数是影响玉米产量的重要因素之一,研究控制玉米雄穗分枝数的 QTL 位点对玉米品种改良、分子辅 助育种具有重要意义。本研究利用京科 968 的双亲京 724 和京 92 构建 BC1F1 群体,以 Maize6H-60K 高质量高密度 SNP 芯 片鉴定群体基因型,获得 28910 个高质量多态性 SNP,构建了包含 2737 个 BIN 标记的高密度遗传图谱,各染色体 BIN 标记 数在 145~512 个之间,BIN 标记平均遗传距离为 0.56 cM。2021 年将亲本及 727 个单株种植在北京,调查雄穗分枝数。采用 QTL IciMappingV4.2 的完备区间作图法进行雄穗分枝数 QTL 检测及定位,共检测到 6 个 QTL,分别位于第 2、5、6、7、8 和 9 染色体,QTL 的 LOD 值范围为 3.18~11.08,揭示 1.58%~5.59% 的表型变异。通过物理位置比对,6 个 QTL 中有 4 个与前人 定位在相同区域,qTBN6 和 qTBN7 尚未见报道。其中 qTBN6 的 LOD 为 6.73,增效等位基因来自京 724,具有负的加性效 应,作用为减少雄穗分枝数。本研究为克隆调控雄穗分枝数功能基因奠定了基础。

    Abstract:

    The tassel branch number of maize is one of the important factors affecting the yield production. Unlocking the QTL loci to control the tassel branch number of maize is of great significance for maize variety improvement and molecular assisted breeding. In this study,the individuals of a BC1F1 population derived from the parents Jing724 and Jing92 of Jingke968 were genotyped using high-throughput high-quality SNP array Maize6H-60K. By taking use of 28910 polymorphic SNPs,a high density genetic map containing 2737 BIN markers was constructed. The number of BIN markers on each chromosome ranged from 145 to 512, and the average genetic distance was 0.56 cM. Both parents and 727 offspring lines were planted in Beijing in 2021 to investigate the tassel branch number,followed by QTL mapping using Inclusive Composite Interval Mapping(ICIM) in software QTLIciMappingV4.2. A total of 6 QTL were detected,located on chromosomes 2,5,6,7,8 and 9,respectively. The LOD score of QTL ranged from 3.18 to 11.08,revealing 1.58% to 5.59% phenotypic variation. While four QTL were located in the same or adjacent area as previously reported,two novel QTL qTBN6 and qTBN7 have been revealed. The LOD of qTBN6 was 6.73,and the synergistic allele derived from Jing724,with a negative additive effect to reduce the tassel branch number. Collectively, this study laid a foundation for future isolating the functional genes that regulate the tassel branch number in maize.

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徐 翠,许理文,葛建镕,等.利用高密度SNP芯片定位玉米雄穗分枝数QTL[J].植物遗传资源学报,2022,23(3):832-841.

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  • 收稿日期:2021-12-23
  • 最后修改日期:2022-01-09
  • 录用日期:2022-02-08
  • 在线发布日期: 2022-05-10
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