大豆长链非编码RNA lncRNA77580转基因材料的转录组分析
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作者单位:

1.中国农业科学院作物科学研究所;2.中央民族大学生命与环境科学学院

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基金项目:

国家自然基金(31601302);国家重点研发计划项目(2016YFD0100304);中国农业科学院科技创新工程项目;江苏省现代作物生产协 同创新中心;现代作物生产省部共建协同创新中心


Transcriptome Analysis of Soybean lncRNA77580 Transgenic Hairy Root
Author:
Affiliation:

1.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences;2.College of Life and Environmental Sciences,Minzu University of China

Fund Project:

National Natural Science Foundation of China(31601302),National Key Research and Development Program of China (2016YFD0100304),Agricultural Science and Technology Program for Innovation Team of CAAS,Jiangsu Collaborative Innovation Center for Modern Crop Production,Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry

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    摘要:

    长链非编码 RNAs(lncRNAs,long non-coding RNAs)是长度在 200 个核苷酸以上的功能性 RNA 分子,在植物 的生长发育及逆境应答中发挥重要调控功能。前期研究发现一个大豆盐胁迫响应 lncRNA,称其为 lncRNA77580,它的大片 段缺失和过表达影响其邻近蛋白编码基因的表达。本研究通过 lncRNA77580 转基因材料的转录组分析,探究 lncRNA77580 可能的功能及调控网络。在大豆发状根中过表达 lncRNA77580,转基因复合体表现为盐敏感;利用 dual-sgRNA/Cas9 成功 实现 lncRNA77580 大于 4 kb 的大片段敲除,突变效率为 12.5%。利用 lncRNA77580 转基因材料进行 RNA-seq 分析发现, lncRNA77580 过表达和大片段缺失显著改变了转基因发状根的基因表达谱。差异表达基因(DEGs,differentially expressed genes)GO 和 KEGG 富集分析表明,lncRNA77580 过表达或敲除主要影响了大量转录因子和信号传导类基因的差异表达,这 些差异表达基因与植物逆境应答及生长发育密切相关。上述结果说明 lncRNA77580 可能是在大豆逆境胁迫响应及生长发育 过程中具有重要功能的一个长链非编码 RNA。

    Abstract:

    Long non-coding RNAs(lncRNAs)are functional RNA molecules with>200 nucleotides in length,and play an important regulatory role in the growth and development of plant. We previously identified a salt stress-related lncRNA in soybean,called lncRNA77580. Deletion and overexpression of lncRNA77580 were found to alter the expression of several neighboring protein-coding genes. In this study,we explored the function and regulation network of lncRNA77580 by the transcriptome analysis of lncRNA77580 transgenic hairy root. Over-expressing lncRNA77580 in soybean hairy roots showed salt sensitive. We generated the edited lines harboring>4kb fragment deletion in lncRNA77580 using dual-sgRNA/Cas9 technology(mutation efficiency:12.5%). By conducting the transcriptome sequencing on the hairy roots with lncRNA77580 overexpressing or knockout,significant changes on transcriptional profiles of the transgenic hairy roots were detected. GO enrichment and KEGG pathway analysis of differentially expressed genes(DEGs)showed that overexpressing or knockout of lncRNA77580 had modified the transcriptional profiles in a large number of transcription factors and signaling genes,which are annotated to be related with plant stress response and plant growth and development. Collectively,these results suggested that lncRNA77580 is a long non-coding RNA with important functions in stress response of soybean and plant growth and development.

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牛风娟,陈向前,高 飞,等.大豆长链非编码RNA lncRNA77580转基因材料的转录组分析[J].植物遗传资源学报,2022,23(3):842-856.

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  • 收稿日期:2021-12-08
  • 最后修改日期:2021-12-31
  • 录用日期:2021-12-31
  • 在线发布日期: 2022-05-11
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