割手密干旱响应NAC转录因子的克隆及表达分析
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云南农业大学农学与生物技术学院

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国家重点研发计划项目(2018YFD1000503);国家自然科学基金(31960451);云南省作物生产与智慧农业重点实验室专项 (202105AG070007);云南省教育厅科学研究基金项目(2020Y203)


Cloning and Expression Analysis of Drought Responsive NAC Transcription Factor Gene in Saccharum spontaneum L.
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College of Agronomy and Biotechnology,Yunnan Agricultural University

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National Key Research and Development Program of China(2018YFD1000503),National Natural Science Foundation of China(31960451),Special Projects of the Key Laboratory for Crop Production and Smart Agriculture of Yunnan Province (202105AG070007),Scientific Research Fund Project of Department of Education Yunnan Provincial(2020Y203)

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    摘要:

    NAC 转录因子参与植物对生物和非生物胁迫响应过程。本研究以甘蔗野生种割手密为材料,克隆得到 3 个 SsNACs 基因,命名 SsNAC2、SsNAC3、SsNAC4。生物信息学分析表明,其编码序列 CDS 全长 931 bp、486 bp、781 bp,编码 309 个、162 个、259 个氨基酸。亚细胞定位预测 3 个 SsNACs 基因位于细胞核,上游启动子含有与逆境胁迫应激相关的 ABRE、 LTR、MBS、MYB、STRE 顺式作用元件。系统进化分析表明 SsNAC2 与高粱 SbNAC68 亲缘关系最近,属于 OsNAC3 亚家族, SsNAC3 与南荻 MlNAC1 亲缘关系最近,属于 ATAF 亚家族,SsNAC4 与高粱 SbNAC82 同属 NAC2 亚家族。组织特异性表达 分析结果 SsNAC2、SsNAC3 基因在割手密茎叶生长期表达量较高。基因表达谱分析表明,不同胁迫下 3 个 SsNACs 基因的相 对表达水平不同,推测 SsNAC2、SsNAC3 和 SsNAC4 基因可能参与调控割手密应对干旱、低温、盐分、病原真菌等非生物和生 物胁迫,同时又能够被 ABA 和 MeJA 诱导表达,以上研究为进一步分析 NAC 转录因子在割手密响应逆境胁迫中的功能奠定 理论基础。

    Abstract:

    NAC transcription factors are known to be involved into responses to biotic and abiotic stresses in plants. In this study,we cloned by sequence homology 3 SsNAC genes from sugarcane wild species S. spontaneum L.,named as SsNAC2,SsNAC3 and SsNAC4. Bioinformatic analysis showed that the full-length coding sequences of SsNAC2,SsNAC3 and SsNAC4 were 931 bp,486 bp,and 781 bp,encoding 309,162 and 259 amino acids,respectively. The subcellular location prediction indicated that the deduced proteins of SsNAC2, SsNAC3 and SsNAC4 were localized in the nucleus. The promoters of SsNAC2,SsNAC3 and SsNAC4 were predicted with putative stress-responsive cis-acting elements including an ABRE,LTR,MBS,MYB and STRE. Phylogenetic tree analysis showed that SsNAC2 within the OsNAC3 subgroup was closely related to Sorghum SbNAC68 gene. SsNAC4 and its homolog gene sorghum SbNAC82 belonged to NAC2 subgroup,and SsNAC3 in the ATAF subgroup was closely related to Miscanthus MlNAC1. The tissue-specific analysis showed that SsNAC2 and SsNAC3 were highly expressed in stem and leaves. The transcription profile analysis showed that SsNAC2, SsNAC3 and SsNAC4 were variable on expression under different stress conditions. It′s speculated that SsNAC2, SsNAC3 and SsNAC4 might be involved in regulation of abiotic and biotic stresses including drought,low temperature,salinity,pathogenic fungi,etc. The transcripts of SsNAC2,SsNAC3 andSsNAC4 were inducible by ABA and MeJA treatments. These results provided theoretical basis for future deciphering the functions of NAC genes under stresses conditions in S. Spontaneum L.

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沈庆庆,钱禛锋,谷书杰,等.割手密干旱响应NAC转录因子的克隆及表达分析[J].植物遗传资源学报,2022,23(3):906-916.

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  • 收稿日期:2021-12-05
  • 最后修改日期:2021-12-21
  • 录用日期:2021-12-30
  • 在线发布日期: 2022-05-11
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