High sugar content germplasm is the basic resource for succeeding the breeding of high sugar varieties in sugarcane（Saccharum officinarum L.），and evaluation of its genetic variation is important for improving the breeding efficiency. In this study，the variance and genetic parameters（genetic variance，broadsense heritability and coefficient of variation and so on）in sucrose content were evaluated using 292 germplasm entries，based on three years（one plant and two ratoon crops）field experiments. The sucrose content among the genotypes were significantly different. The interaction between years and genotypes was not significant，and a high broad-sense heritability（0.85，based on 3-year trial）was found. A considerably broader variation with a mean of 10.66% was observed，while the coefficient of variance（CV）was relatively low with an average of 10.13%. That was possibly caused by the constitution of the germplasm involved in this study as most of the entries were released cultivars. Moreover，we found that CV was highest in the month of November regardless of plant or ratoon crops，and the phenotypic and genetic correlation coefficient reached to 0.94 and 0.95（P<0.001）， respectively，implying that selection in November or earlier may be good for sucrose content selection. As a result，32 genotypes with high sugar content（more than 16.00%）were obtained and qualified as high sugar parental germplasm resource for sugarcane breeding. We further proposed some suggestions for high sugar breeding in sugarcane.