不同树龄枸杞古树的遗传多样性研究
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作者单位:

1.宁夏大学生命科学学院;2.宁夏林业和草原局宁夏枸杞产业发展中心;3.宁夏中宁县杞鑫枸杞种业有限公司;4.北方民族大学生物科学与工程学院

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基金项目:

宁夏回族自治区自然科学基金(2021AAC02005,2022AAC05036,2020AAC03097);国家自然科学基金(81760682);宁夏枸杞产业发展中 心项目(022004089013)


Genetic Diversity Analysis of Ancient Trees with Different Ages of Lycium barbarum L.
Author:
Affiliation:

1.College of Life Sciences,Ningxia University;2.Ningxia Forestry and Grassland Bureau Ningxia Lycium barbarum L Industry Development Center;3.Ningxia Zhongning County Qixin Lycium barbarum L Seed Industry Limited Company;4.School of Biology and Engineering, North University for Nationalities

Fund Project:

Ningxia Hui Autonomous Region Natural Science Foundation(2021AAC02005,2022AAC05036,2020AAC03097),National Natural Science Foundation of China(81760682),Ningxia Wolfberry Industry Development Center Program

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    摘要:

    通过对 43 份枸杞材料进行表型性状指标的测定,结合 SSR 分子标记技术,综合分析枸杞古树与现有宁夏枸杞品 种(系)之间的群体结构和遗传多样性。表型性状主成分分析结果确定了主成分为 4,累计贡献率达到 78.09%,第一主成分叶 片大小和第二主成分果实大小起主要贡献作用。样品变异系数范围为 15%~54%,平均值为 26.78%,样品遗传变异指数范围为 5.26~5.41。表型性状指标通过聚类分析后分为 4 个类群,第Ⅰ类群 3 份材料,第Ⅱ类群 3 份材料,第Ⅲ类群 8 份材料,第Ⅳ类群 29 份材料。采用隶属函数法分析确定综合性状优良的 5 个材料,分别为 L14、 L16、L28、L40、L43。SSR 分子标记遗传信息有效 等位基因平均值为 2.018,期望杂合度平均值为 0.398,Shannon′s 指数平均值为 0.839。SSR 分子标记确定最优 K 值为 4,综合评价 较好的 5 份材料中,L16 表现为杂合基因型,L14、L28、L40、L43 表现为单一基因型。本研究运用多种方法综合分析了不同来源、 不同树龄枸杞古树的表型性状差异和遗传多样性,为后续枸杞古树种质资源的利用及枸杞品种选育提供了理论依据和实践指导。

    Abstract:

    Through the determination of phenotypic traits of 43 wolfberry materials,combined with SSR molecular marker technology,the population structure and genetic diversity between ancient wolfberry trees and existing wolfberry varieties in Ningxia were comprehensively analyzed. The principal component analysis results of phenotypic traits determined that the principal component was 4,and the cumulative contribution rate reached 78.09%. The leaf size of the first principal component and the fruit size of the second principal component played a major role. The coefficient of variation ranged from 15% to 54%,with an average of 26.78%,and the genetic variation index ranged from 5.26 to 5.41. The phenotypic traits were divided into 4 groups by cluster analysis,3 individuals in group I,3 individuals in group II,8 individuals in group III and 29 individuals in group IV. Five materials with excellent comprehensive properties were determined by membership function method,namely L14,L16,L28,L40 and L43. The average effective alleles of SSR molecular markers were 2.018,the average expected heterozygosity was 0.398,and the average Shannon ′ s index was 0.839. The optimal K value of SSR molecular markers was 4. Among the five materials with better comprehensive evaluation,L16 showed heterozygous genotype,and L14,L28,L40 and L43 showed single genotype. In this study,a variety of methods were used to comprehensively analyze the phenotypic traits and genetic diversity of ancient wolfberry trees from different sources and ages,which provided a theoretical basis and practical guidance for the subsequent utilization of ancient wolfberry germplasm resources and the breeding of wolfberry varieties.

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引用本文

胡永超,马 洁,唐建宁,等.不同树龄枸杞古树的遗传多样性研究[J].植物遗传资源学报,2022,23(3):755-767.

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  • 收稿日期:2021-11-05
  • 最后修改日期:2021-12-03
  • 录用日期:2021-12-17
  • 在线发布日期: 2022-05-10
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