杜梨对盐碱胁迫的生理响应及耐盐碱性评价
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中国农业科学院果树研究所

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中国农业科学院科技创新工程(CAAS-ASTIP);财政部和农业农村部:国家现代农业产业技术体系(CARS-28-1)


Physiological Response and Saline-alkali Tolerance Evaluation of Pyrus betulifolia Resources under Saline-alkali Stress
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Research Institute of Pomology,Chinese Academy of Agricultural Sciences

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The Agricultural Science and Technology Innovation Program(CAAS-ASTIP),China Agriculture Research System of MOF and MARA(CARS-28-1)

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    摘要:

    为探究不同杜梨资源对盐碱胁迫的生理响应及筛选耐盐碱资源,以甘肃、山西、辽宁等地收集的 17 份杜梨资源 3000 余株幼苗为试验材料,采用中性盐和碱性盐混合胁迫处理,测定盐碱抗性相关的生理指标,基于主成分分析法、热图 及聚类分析法和抗逆系数法进行抗性评价。研究发现,抗性强的资源抗氧化酶活性和脯氨酸含量在受到胁迫后显著上升, 300 mmol/L 盐碱胁迫下超氧化物歧化酶活性显著上升的资源为河底杜梨 3 和平陆杜梨 1,分别上升了 75.04%、70.04%;过氧 化物酶活性上升显著的资源为甘肃杜梨 1 和河底杜梨 1,分别上升了 1136.11%、742.86%;过氧化氢酶活性显著上升的资源为 甘肃杜梨 15、甘肃杜梨 4,分别上升了 344.58%、343.42%,脯氨酸含量显著上升的资源为平陆杜梨 2、河底杜梨 1、河底杜梨 3, 丙二醛含量上升较多的资源为甘肃杜梨 16、甘肃杜梨 13、甘肃杜梨 1。除甘肃杜梨 13 在低浓度(100 mmol/L)盐碱胁迫即出 现明显盐害现象外,其他杜梨资源均在 300 mmol/L 浓度处理下才开始出现不同程度的盐害;利用不同方法进行耐盐碱性分 析结果存在一定的差异,其中基于主成分分析法发现河底杜梨 1、河底杜梨 3、平陆杜梨 1、甘肃杜梨 15、山西杜梨 1 耐盐碱性 强;基于热图和聚类分析发现甘肃杜梨 4、甘肃杜梨 15、平陆杜梨 1、河底杜梨 1 和河底杜梨 3 具有较强的耐盐碱性;而利用抗 逆系数法分析耐盐碱性较强的资源为甘肃杜梨 1、河底杜梨 1、甘肃杜梨 16、甘肃杜梨 4、甘肃杜梨 15。综合 3 种方法的分析 结果,河底杜梨 1 和甘肃杜梨 15 属于高抗盐碱类型,而甘肃杜梨 13 则为盐碱敏感型资源。

    Abstract:

    To unlock the physiological responses of P. betulaefolia Bunge germplasm resources and identify the elite resources under saline-alkali stress conditions,>3000 seedling plants from 17 betulaefolia Bunge accessions in Gansu,Shanxi and Liaoning provinces were analyzed. The physiological indexes under the mixed stress treatments of neutral salt and alkaline salt were measured,followed by statistical analysis(principal component analysis,heat map,cluster analysis and stress coefficient). In P. betulaefolia Bunge plants showing strong tolerance,the antioxidant enzyme activity and proline content increased significantly after treatments. Under 300 mmol/L salt-alkali stress,the SOD activity in P. betulaefolia-Hedi 3 and P. betulaefolia-Pinglu 1 was observed with increase up to 75.04% and 70.04%,respectively. The increase on the POD activity in P.betulaefolia-Gansu 1 and P. betulaefolia-Hedi 1 was detected up to 1136.11% and 742.86%,respectively. In P. betulaefolia-Gansu 15 and P. betulaefolia-Gansu 4 a significant increase(344.58% and 343.42%,respectively) on the CAT activity was detected. Moreover,the significant increase on proline content were observed in P. betulaefolia-Pinglu 2,P. betulaefolia-Hedi 1 and P. betulaefolia-Hedi 3 and MDA content were observed in P. betulaefolia-Gansu 16,P. betulaefolia-Gansu 13 and P. betulaefoli-Gansu 1. Except for P. betulaefolia-Gansu 13 that showed salt damage at low concentration(100 mmol/L)under saline-alkali stress,other P. betulaefolia Bunge resources showed degrees of salt damage under 300 mmol/L. By deployment of the principal component analysis,P. betulaefolia-Hedi 1,P.betulaefolia-Hedi 3,P. betulaefolia-Pinglu 1,P. betulaefolia-Gansu 15 and P. betulaefolia-Shanxi 1 showed strong salt-alkali resistance. Based on heat map and cluster analysis,it was found that P. betulaefolia-Gansu 4,P. betulaefolia-Gansu 15,P. betulaefolia-Pinglu 1,P. betulaefolia-Hedi 1 and P. betulaefolia-Hedi 3 had strong salinity tolerance. The coefficient analysis suggested that P. betulaefolia-Gansu 1,P. betulaefolia-Hedi 1,P. betulaefolia-Gansu 16,P. betulaefolia-Gansu 4,P. betulaefolia-Gansu 15 showed strong salt and alkali tolerance. Taken together,P. betulaefolia Bunge. P. betulaefolia-Hedi 1 and P. betulaefoliaGansu 15 are qualified with high saline-alkali resistance,whereas P. betulaefolia-Gansu 13 is saline-alkali sensitive.

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引用本文

霍宏亮,王 超,杨 祥,等.杜梨对盐碱胁迫的生理响应及耐盐碱性评价[J].植物遗传资源学报,2022,23(2):480-492.

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  • 收稿日期:2021-09-18
  • 最后修改日期:2021-10-29
  • 录用日期:2021-11-23
  • 在线发布日期: 2022-03-09
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