山西小麦地方品种品质相关基因的KASP标记分析
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山西农业大学农业基因资源研究中心 / 农业部黄土高原作物基因资源与种质创制重点实验室 / 杂粮种质资源发掘与遗传改良山西省重点实验室

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山西省面上青年基金项目(201801D221313);山西省农业科学院农业科技创新研究课题(YCX2020YQ48);山西省重点研发计划 项目(201903D211003)


Detection of Quality-Related Genes in the Wheat Landrace in Shanxi Province by KASP Markers
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Affiliation:

Center for Agricultural Genetic Resources Research,Shanxi Agricultural University/Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture/Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops

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Shanxi Province General Youth Fund Project(201801D221313),Shanxi Academy of Agricultural Sciences Agricultural Science and Technology Innovation Research Project(YCX2020YQ48),Key Research and Development Projects in Shanxi Province(201903D211003)

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    摘要:

    为发掘和利用小麦地方品种中的优良品质性状基因资源,本研究利用 30 个 KASP 分子标记对 405 份山西小麦地 方品种的 15 个品质性状相关基因进行了检测。结果表明,在供试材料中籽粒硬度优异等位变异 Pinb-D1b 与 Pinb-B2b 分别占 比为 4.94% 和 63.46%;麦谷蛋白 Glu-1 位点的优异等位变异 Glu-Ax1 或 Glu-Ax2* 占比为 93.09%,Glu-Bx7OE 占比为 47.16%, Glu-D1d 占比为 0.74%;面粉色泽相关的优异等位变异 Psy-A1b 占比为 1.23%,Psy-B1a 或 Psy-B1b 为 95.80%,Psy-D1a 为 87.65%,TaPds-B1b 为 0.74%,Zds-A1a 为 87.16%,TaLcy-B1b 为 96.05%,Lox-B1a 为 88.89%,TaPod-A1b 为 1.73%,Ppo-A1b 为 97.28%,Ppo-D1a 为 20.94%。且在山西中部晚熟和南部中熟冬麦区中优异等位变异的频率分布范围分别为 0.52%~97.41%、 0~97.51%。此外,鉴定出 89 份材料聚合 9 个优异等位变异的组合 Pinb-B2b/Glu-Ax1 或 Glu-Ax2*/Glu-Bx7OE/Psy-B1a 或 PsyB1b/Psy-D1a/Zds-A1a/TaLcy-B1b/Lox-B1a/Ppo-A1b,其频率为 21.98%。本研究筛选出含有优异等位变异的小麦地方品种,为 小麦品质相关性状的遗传改良提供了重要参考。

    Abstract:

    To explore and utilize the genetic resources of good quality traits in wheat landraces,15 quality trait-related genes in 405 wheat landraces of Shanxi province were genotyped using 30 KASP markers. The results showed that the average frequency of the favorable allelic variations Pinb-D1b and Pinb-B2b for grain hardness accounted for 4.94% and 63.46%,respectively. At the Glu-1 locus,the favorable alleles Glu-Ax1(or Glu-Ax2* ),Glu-Bx7OE and Glu-D1d accounted for 93.09%,47.16% and 0.74%,respectively. Elite alleles for flour color including, Psy-A1b,Psy-B1a(or Psy-B1b),Psy-D1a,TaPds-B1b,Zds-A1a,TaLcy-B1b,Lox-B1a,TaPod-A1b,Ppo-A1b and Ppo-D1a accounted for 1.23%,95.80%,87.65%,0.74%,87.16%,96.05%,88.89%,1.73%,97.28% and 20.94%, respectively. The favorable allele frequncy of landraces,which were derived from the late-maturing winter wheat region in central Shanxi and the south mid-maturing winter wheat region,ranged from 0.52% to 97.41% and 0 to 97.51%,respectively. In addition,nine favorable alleles Pinb-B2b/Glu-Ax1(or Glu-Ax2* )/Glu-Bx7OE/Psy-B1a (or Psy-B1b)/Psy-D1a/Zds-A1a/TaLcy-B1b/Lox-B1a/Ppo-A1b were simultanously detected in 89 samples,with a frequency of 21.98%. By conducting marker-assisted identification of the excellent alleles in wheat landraces,this study provided reference for the genetic improvement at quality-related traits in bread wheat.

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刘 霞,雷梦林,王艳珍,等.山西小麦地方品种品质相关基因的KASP标记分析[J].植物遗传资源学报,2022,23(3):881-894.

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  • 收稿日期:2021-09-10
  • 最后修改日期:2022-01-03
  • 录用日期:2022-01-28
  • 在线发布日期: 2022-05-11
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