1.河南科技学院 小麦中心;2.河南科技学院新科学院
河南省科技攻关项目(202102110161);河南省高校重点科研项目(21A210007)
1.Center of Wheat Research, Henan Institute of Science and Technology;2.Xinke College, Henan Institute of Science and Technology
The Key projects of Science and Technology of Henan Province (No.202102110161 );the Key Scientific Research Projects of Colleges and Universities in Henan Province (No.21A210007)
为有效利用外引小麦种质资源,本研究对收集的47份外引小麦种质材料进行Waxy和HMW-GS等位基因的分子检测,并分析了其直链淀粉、支链淀粉、湿面筋等品质参数。结果表明,在Wx-A1位点存在3种类型:Wx-A1a、Wx-A1g和Wx-A1b,39份材料(82.98%)以Wx-A1a类型为主;Wx-B1位点3种类型:Wx-B1a、Wx-B1e和Wx-B1b,37份材料(78.72%)以Wx-B1a类型为主;Wx-D1位点2种类型Wx-D1a和Wx-D1b,46份材料具有Wx-D1a类型;共鉴定出8种Wx-1位点等位基因组合,31份材料(65.96%)以Wx-A1a/B1a/D1a为主。在Glu-A1位点,含有等位基因Ax2*、Null和Ax1类型的材料分别为18份、18份和11份;在Glu-D1位点,含有等位基因Dx2和Dx5类型的材料分别为23份(48.94%)和21份(44.68%),含有等位基因Dy12和Dy10类型的材料分别为22份(46.81%)和20份(42.55%),具有Dy10+Dy12类型材料2份;共鉴定出19种Glu-A1/D1等位基因组合,7份材料含有Null/Dx5+Dy12。含有Wx-A1a/B1a/D1a材料的直链淀粉含量相对较高,支链淀粉含量相对较低;含有优质等位基因Ax1或Ax2*兼Dx5+Dy10材料的湿面筋含量相对较高。总体上这些外引种质资源Waxy和HMW-GS等位基因类型丰富,为种质资源合理利用和现代普通小麦品质改良提供参考依据。
In order to effectively utilize wheat germplasm introduced from abroad, Waxy and HMW-GS alleles were detected in 47 introduced wheat materials, and the quality parameters of amylose, amylopectin and wet gluten were analyzed. The results showed that there were three types of Wx-A1a, Wx-A1g and Wx-A1b at the Wx-A1 locus, and 39 materials (82.98%) were mainly of Wx-A1a type. Wx-B1a, Wx-B1e and Wx-B1b were located in the Wx-B1 locus, and 37 materials (78.72%) were mainly of Wx-B1a type. Wx-D1a and Wx-D1b were discovered at Wx-D1 locus, and 46 materials were of Wx-D1a type. A total of 8 Wx-1 allele combinations were identified, and 31 materials (65.96%) were mainly of Wx-A1a/B1a/D1a combination. The results also showed that the Glu-A1 alleles Ax2*, Null and Ax1 were distributed in 18, 18 and 11 materials, respectively. At the Glu-D1 locus, it was found that 23 (48.94%) and 21 (44.68%) materials contained Dx2 and Dx5, respectively; 22 (46.81%) and 20 (42.55%) materials contained Dy12 and Dy10, respectively; 2 materials possessed heterozygous Dy10+Dy12 type. A total of 19 kinds of Glu-A1/D1 allele combinations were identified, and Null/Dx5+Dy12 allele combinations were displayed in 7 materials. The quality analysis showed that the materials with Wx-A1a/B1a/D1a had relatively high amylose and low amylopectin contents, and the materials with Ax1 or Ax2* and Dx5+Dy10 possessed relatively high wet gluten content. Those results suggested that the introduced wheat germplasms were rich in Waxy and HMW-GS alleles, which would provide reference for rational utilization of germplasm resources and quality improvement of modern common wheat.
胡喜贵,吴晓军,张海惠,等.47份外引小麦种质资源Waxy和HMW-GS分子检测与品质性状分析[J].植物遗传资源学报,2021,22(2):371-379.
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