大豆高蛋白种质中引1106蛋白质含量的QTL分析
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作者单位:

1.长江大学农学院;2.黑龙江省农业科学院黑河分院;3.黑龙江省农业科学院大豆研究所;4.农作物基因资源与遗传改良国家重大科学工程/农业部北京大豆生物学重点实验室/中国农业科学院作物科学研究所

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基金项目:

中国农科院创新工程基金项目;黑龙江省农业科学院院级科研项目(2017XQ04);湿地生态与农业利用教育部工程中心开放基金(KF201808)


QTL Mapping of Soybean Protein Content from High-Protein Soybean ZhongYin1106
Author:
Affiliation:

1.College of Agriculture, Yangtze University,Hubei Jingzhou;2.Soybean Research Center, Heihe Institute, Heilongjiang Academy of Agricultural Sciences;3.Soybean Research Institute, Heilongjiang Academy Agricultural of Science;4.National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Soybean Biology in Beijing (MOA) / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences

Fund Project:

Innovation project of Chinese Academy of Agricultural Sciences; Scientific Research project of Heilongjiang Academy of Agricultural Sciences(2017XQ04); open fund of Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education (KF201808)

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    摘要:

    大豆籽粒蛋白质含量由多基因控制且易受环境条件的影响,发掘高蛋白基因是促进大豆优质分子育种的重要手段。本研究选用综合性状优良、蛋白质含量较低的黑河50为轮回亲本,以引进的高蛋白种质中引1106为供体亲本,构建了由384个家系组成的回交高代群体。利用近红外光谱仪测定回交群体的蛋白质含量,使用SSR分子标记技术鉴定回交群体BC1F6基因型,通过QTL ICIMapping4.1的区间作图法(IM-ADD)和完备区间作图法(ICIM-ADD)定位蛋白含量QTL,共获得9个蛋白含量QTL,其中IM定位到7个蛋白含量QTL,而ICIM定位到3个蛋白含量QTL,两种方法同时在8号染色体上定位到一个QTL(qPro-8-1),该QTL两侧的分子标记是SSR_50和SSR_51,可解释表型变异分别是2.26%和7.85%,定位区间物理距离大小为218.71 kb,该QTL尚未见报道,是一个与蛋白质含量相关的新QTL位点,为高蛋白大豆品种选育提供了材料和理论依据。

    Abstract:

    The protein content in soybean is genetically controlled by multiple genes. Identification of the quantitative traits (QTL) associating with this trait will benefit for a practice use in soybean breeding. In this study, a backcross population consisting of 384 lines was derived from a cross of elite cultivar Heihe50 as recurrent parent and high protein accession ZhongYin1106 as donor parent. The protein content of parents and the offspring segregants was detected by Near-infrared spectrometer, and genotyping was conducted with SSR molecular markers. A genetic linkage map was constructed by software QTL ICIMapping4.1. Nine QTL associating with protein content were revealed, including seven QTL detecting by interval mapping (IM) and 3 QTLs detecting by inclusive composite interval mapping (ICIM). One QTL qPro-8-1, which was located in a 218.71kb interval of markers SSR_50 and SSR_51, can explain 2.26% and 7.85% phenotype variance using both IM and ICIM mapping methods, respectively. The locus qPro-8-1 is genetically independent of other QTL of protein content. Thus, this work provided elite materials and theoretical foundation in molecular breeding for high-protein soybean varieties.

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引用本文

王婉,韩德志,闫洪睿,等.大豆高蛋白种质中引1106蛋白质含量的QTL分析[J].植物遗传资源学报,2020,21(1):130-138.

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  • 收稿日期:2019-06-06
  • 最后修改日期:2019-06-12
  • 录用日期:2019-06-24
  • 在线发布日期: 2020-01-17
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