Main agronomic characteristics of 12451 (repetition eliminated) rice germplasm resources collected and conserved in Hunan plant gene bank during 1992-2012 were analyzed and evaluated. The results showed those germplasm resources showed significant regional characteristics. Different types of rice germplasm resource had their own unique characteristics. 75.43% of the germplasm was utilized. Moreover, some suggestions were put forward for the collection, conservation, evaluation and utilization of rice germplasm resources.