Identification of leaf rust resistance genes is important for developing leaf rust resistant wheat cultivars. A total of 104 Xinjiang winter wheat cultivars and advanced lines were inoculated with 12 Chinese pathotypes of Puccinia triticina for postulation of leaf rust resistance genes effective at the seedling stage. These genotypes were also planted in the field for characterization of slow rusting responses to leaf rust in the 2007-2008 and 2008-2009 cropping seasons. Six leaf rust resistance genes Lr1, Lr3ka, Lr14a, Lr26, Lr34, and Lr50, either singly or in combinations, were found in 41 genotypes, whereas known resistance genes were not identified in the other 63 accessions. Resistance gene Lr26 was present in 21 accessions. Lr34 was identified in 17 lines. Genes Lr1 and Lr14a were each detected in three entries. Lr3ka was present in two lines and Lr50 may exist in one line. Seven genotypes showed slow leaf rusting resistance in two cropping seasons.