Germplasms with high sugar content in stem are of importance for silage corn breeding. One hundred and fifty one corn inbred lines were screened for evaluation of sugar content in stem in this study. The results were summarized as follows, the Brix in stem ranged from 3.5 to 16.9, 65 out of 151 inbred lines (43.1%) had sugar content up to 10%,and these inbred lines might be useful for improvement of sugar content of hybrid stem. All the tested inbred lines were divided into three types using cluster analysis method according to their sugar content in stem. Type III included 22 inbred lines and these lines had high sugar content of stem(Brix=12.3～16.9),which might be pivotal to increase sugar content of corn hybrid stem. Five out of these 22 lines including 78599-1-550, 78599-2, YXD053-646, Y53-245 and YU-CY509 could be desirable genotypes considering their high sugar content in stem and superior agronomic characters.