In this study, 300 soybean and 100 common bean germplasm conserved in National Crop GeneBank were screened for seed-borne viruses by serological and molecular biological methods. For the soybean germplasms, 76(25.33%), 41(13.67%) and 14 (4.67%) were positive to Soybean mosaic virus (SMV), Cucumber mosaic virus (CMV), and Alfalfa mosaic virus (AMV) respectively. Some were infected by two viruses, such as SMV and CMV, or SMV and AMV. In common bean 92% were infected by Bean common mosaic virus (BCMV). These information will be useful to understand the status of seed-borne viruses in leguminous crops in genebank and to take measures for control virus diseases during seed reproductions in field.