A set of materials with Triticum aestivum- Haynaldia villosa translocation chromosomes were created using cv. ‘Chinese Spring’ as female and Triticum durum-Haynaldia villosa amphiploid as male whose pollen treated with 60Co-γ-ray in different dose. Then the set of materials were backcrossed with cv. ‘Chinese Spring’ or self-crossed , H. villosa chromosome segments were reserved in M1 or BC1 so that alien genes were transferred into wheat. The results showed that frequency of induced translocation chromosomes were significant different using different irradiation doses of 60Co-γ-ray. The plants with T. aestivum-H. villosa translocations induced by 1200rad and 800rad hold 76.7% and 50.0% in M1 generation, respectively, and better translocations types were induced by 60Co-γ-ray with 1200rad dose. 67.6% of these translocations were passed from M1 to BC1, and 96.4% from BC1 to BC2. Alien whole chromosomes were rapidly lost, some pure translocations were obtained in BC2F2.