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  • 李慧琳,奉斌,于二汝,等.贵州不同香型紫苏挥发油成份分析与评价[J].植物遗传资源学报,2020,21(1):269-274.    [点击复制]
  • LI Hui-lin,FENG Bin,YU Er –ru,et al.Analysis and Evaluation of Volatile Oil Content in Leaves of Different Varieties Perilla frutescens in Guizhou[J].植物遗传资源学报,2020,21(1):269-274.   [点击复制]
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贵州不同香型紫苏挥发油成份分析与评价
李慧琳, 奉斌, 于二汝, 杨航, 杨胜先, 林涛, 魏忠芬
0
(贵州省农业科学院油料(香料)研究所,贵阳 550006)
摘要:
挥发油是紫苏叶片中主要功效成分之一,具有显著的抑菌、消炎、抗氧化作用。本试验以 18 份贵州省香型紫苏种 质资源为研究材料,采用水蒸气蒸馏法提取了紫苏叶片中的挥发油,运用气质联用技术(GC_MS)进行挥发油成分分析。研究结果显示,不同紫苏种质之间挥发油含量及成分差异显著,挥发油得率在 0.46%~1.44%之间。紫苏的挥发性物质成分含量 丰富,共鉴定出 75 种化学成分,主要成分为紫苏酮、胡椒酮、榄香素、紫苏醛、肉豆蔻醚、石竹稀、紫苏烯、芹菜脑、苯乙 酮,按主成分进行分类,可分为 F、PA、PK、PL、PT、PP 6 种化学型。此外还发现几种特殊的含量较少但对香气影响较大 的物质,如柠檬醛、香柠檬烯、桉油精、橙花叔醇等,使贵州紫苏资源呈现特有的芳香味道。表明贵州紫苏精油含量丰富、 类型多样,研究结果可为紫苏的品质评价及临床应用提供理论参考。
关键词:  紫苏  种质资源  挥发油成分  化学类型  评价
DOI:10.13430/j.cnki.jpgr.20191105003
投稿时间:2019-11-05修订日期:2019-11-28
基金项目:国家自然科学基金项目(31560403);贵州省人才基地(RCJD2018-14);黔农科院种质资源([2018]02号)
Analysis and Evaluation of Volatile Oil Content in Leaves of Different Varieties Perilla frutescens in Guizhou
LI Hui-lin, FENG Bin, YU Er –ru, YANG Hang, YANG Sheng-xian, LIN Tao, WEI Zhong-fen
(Institute of Oil/Spices Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550006)
Abstract:
Volatile oil, as one of the important functional components in Perilla frutescens leaves, plays a significant role in anti-bacteria, anti-inflammatory and anti-oxidation. In this study, by taking use of 18 perilla germplasm resources in Guizhou province, we extracted the volatile oil with the steam distillation followed by GC-MS analysis. The significant differences in essential oil content and composition among different P. frutescens materials were observed. For instance, the content of essential oil was ranged from 0.46% and 1.44%. Seven-five chemical constituents were identified in the essential oils, mainly including: perillaketone, piperidone, elemicin, perillaldehyde, myristicin, caryophyllene, perillane, apioland acetophenone . Based on synthesis pathways, the tested Perilla materials can be classified into six chemotypes including F-type, PA-type, PK-type, PL-type, PT-type, PP-type. In addition, we also identified some special substances, such as citral, citronellene, eucalyptus oil essence, orange flower tertiary alcohol, the presence of which may provide a hypothesis on explaining a unique aroma of some Perilla plants in Guizhou. Therefore, this work showed abundant diversity on content and chemotype composition of P. frutescens resources in Guizhou, and also might provide theoretical reference for quality control and clinical application in the future.
Key words:  Perilla frutescens  germplasm resources  volatile oil composition  chemotype  evaluation

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