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  • 王瑾,李玉荣,程增书,等.高油酸花生品种(系)ahFAD 鉴定及遗传多样性分析[J].植物遗传资源学报,2020,21(1):208-214.    [点击复制]
  • WANG Jin,LI Yu-rong,CHENG Zeng-shu,et al.ahFAD Genotype Identification and Genetic Diversity Analysis of High Oleic Acid Peanut Cultivars[J].植物遗传资源学报,2020,21(1):208-214.   [点击复制]
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高油酸花生品种(系)ahFAD 鉴定及遗传多样性分析
王瑾,李玉荣,程增书,陈四龙,宋亚辉,刘义杰,张朋娟
0
(河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,石家庄 050035)
摘要:
为研究不同亲本来源高油酸品种(系)的遗传差异,选用来源于 5 个高油酸供体亲本的 25 个品种(系)进行分析。 所选材料油酸含量均大于 75%,油酸亚油酸比值大于 10。对控制油酸基因 ahFAD 进行分析,结果表明,所选高油酸品种(系) 的 ahFAD2A 与 ahFAD2B 均为发生突变,ahFAD2A 为 G448A 的替换,ahFAD2B 为 442 A 的插入。698 对 SSR 引物对 25 个品种 (系)进行检测,140 对引物在品种间检测到差异;共检测到 379 个等位变异,遗传多态性指数的变化范围为 0.074~0.728, 平均 0.371;筛选到 17 个多态性丰富,扩增产物稳定的引物,可用于 25 个品种(系)进行鉴定区分。遗传距离分析表明,25 个品种遗传距离在 0.057~0.624 之间,平均为 0.451。对高油酸品种(系)进行聚类分析,在遗传相似性系数 0.745 处,被分 为 11 个类群,类群分布与亲本来源具有一致性,可实现对一些资源或育成品种进行亲本类型的推测,并为花生分子标记辅助 育种提供参考。
关键词:  花生  高油酸  亲本来源  SSR 标记  遗传多样性
DOI:10.13430/j.cnki.jpgr.20191029005
投稿时间:2019-10-29修订日期:2019-12-02
基金项目:河北省自然科学基金(C2018301076);河北省科技计划项目(16226301D);河北省现代农业产业技术体系(HBCT2018090201);基本科 研业务费(2018JBYWF06)
ahFAD Genotype Identification and Genetic Diversity Analysis of High Oleic Acid Peanut Cultivars
WANG Jin, LI Yu-rong, CHENG Zeng-shu, CHEN Si-long, SONG Ya-hui, LIU Yi-jie, ZHANG Peng-juan
(Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences / Laboratory of Crop Genetics and Breeding of Hebei Province, Shijiazhuang 050035)
Abstract:
To study the genetic difference of the high oleic acid peanut cultivars, twenty-five offspring varieties from five high oleic acid parents were investigated by using gene-based and genetic marker-based diversity analysis approaches. The oleic acid content and O/L of 25 peanut varieties were higher than 75% and 10, respectively. By analyzing the full-length sequence of ahFAD genes of these varieties, a nucleotide substitution of G448A was found in FAD2A, while a nucleotide insertion of A at 442 from the translation starting code in FAD2B was identified. Searching for polymorphism in 25 varieties using 698 SSR markers, 140 SSR markers were found to be polymorphic, which produced a total of 379 alleles. The genetic diversity index value ranged from these 25 varieties were distinguishable by using 17 polymorphic SSR markers. The genetic distance ranged from 0.057 to 0.624 with a mean value of 0.451. Eleven groups were revealed when the genetic similar coefficients of 0.745 which was able to distinguish the parents and the offspring varieties. Taken together, the results may become useful in the pedigree analysis of the high oleic acid peanut varieties and the molecular marker assisted selection in peanut breeding.
Key words:  peanut  high oleic acid  parent-of- origin  SSR markers  genetic diversity

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