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  • 吉康娜,郅俊杰,林丹妮,等.基于茄子基因组重测序的InDel标记开发及应用[J].植物遗传资源学报,2019,20(5):1278-1288.    [点击复制]
  • JI Kang-na,ZHI Jun-jie,LIN Dan-ni,et al.Development and Application of Eggplant InDel Markers Based on Whole Genome Re-sequencing Datasets[J].植物遗传资源学报,2019,20(5):1278-1288.   [点击复制]
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基于茄子基因组重测序的InDel标记开发及应用
吉康娜1, 郅俊杰1, 林丹妮1, 颜爽爽1, 田时炳2, 曹必好1, 邱正坤1
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(1.广东省蔬菜工程技术研究中心/华南农业大学园艺学院;2.重庆市农业科学院蔬菜花卉研究所)
摘要:
本研究基于2 份在果实与抗性上存在显著差异的茄子的全基因重测序数据,鉴定出151327 个InDel 位点,随机选择并设计了180 个InDel 标记,利用重测序材料及其杂交一代进行有效性鉴定,结果显示:180 个InDel 标记均可获得PCR扩增产物,其中62 个标记具有多态性,多态率为34.7%。基于InDel 标记聚类分析结果揭示出24 份茄子自交系和143 份茄子品种的遗传多样性。基于2 对InDel 标记的茄子品种纯度鉴定结果与田间鉴定结果相吻合。
关键词:  茄子  分子标记  InDel  种质分析  品种纯度鉴定
DOI:10.13430/j.cnki.jpgr.20190130001
投稿时间:2019-01-30修订日期:2019-03-12
基金项目:国家重点研发项目(2017YFD0101904);国家自然科学(31801863和31672156)
Development and Application of Eggplant InDel Markers Based on Whole Genome Re-sequencing Datasets
JI Kang-na1, ZHI Jun-jie1, LIN Dan-ni1, YAN Shuang-shuang1, TIAN Shi-bing2, CAO Bi-hao1, QIU Zheng-kun1
(1.Guangdong Vegetable Engineering and Technology Research Center/ College of Horticulture, South China Agricultural University;2.The Institute of Vegetable and Flower Research, Chongqing Academy of Agricultural Sciences)
Abstract:
In this study, 151327 of InDels were in silico identified by taking advantage of whole genome re-sequencing of two eggplant (Solanum melongena) lines. The primers targeting 180 InDel loci were developed, which were subjected in tests of two re-sequencing eggplant lines and derived hybrid line. Out of that, sixty-two (34.7%) of total primers were polymorphic between both varieties. In addition, the genetic diversity and specificity of 24 eggplant lines and 143 varieties were investigated by use of these markers. The results from purity test of variety from two InDel markers matched well with those from field experiments.
Key words:  eggplant  molecular marker  InDel  germplasm analysis  purity test

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