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  • 张晨,云岚,李珍,等.新麦草种质的SSR遗传多样性及群体结构分析[J].植物遗传资源学报,2019,20(1):48-59.    [点击复制]
  • ZHANG Chen,YUN Lan,LI Zhen,et al.Genetic Diversity and Structure Analysis in Psathyrostachys Population Using SSR Markers[J].植物遗传资源学报,2019,20(1):48-59.   [点击复制]
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新麦草种质的SSR遗传多样性及群体结构分析
张晨1, 云岚1,2, 李珍1, 王俊1, 郭宏宇1, 盛誉1, 石子英1, 徐学宝1
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(1内蒙古农业大学草原与资源环境学院,呼和浩特 010018;2内蒙古农业大学牧草种质创新与育种研究所草地资源教育部重点实验室,呼和浩特 010018)
摘要:
利用38对SSR引物对来源于不同国家与地区的171份野生和栽培新麦草单株种质进行遗传多样性和群体结构分析。研究结果显示,有效引物在新麦草单株材料间共获得308个位点,多态性位点275个,多态性百分率为89.29%,供试新麦草材料的遗传相似性系数介于0.537~0.899,平均为0.742。群体结构分析、主成分分析与NJ聚类法均将供试材料分为3个类群,且类群间的材料相互渗透形成混合类群,分类结果与遗传相似性系数分析表明171份新麦草单株种质分布较集中;3种分类方法对新麦草材料3个不同类群的划分结果基本一致,但混合类群的划分存在差异,每个类群的新麦草单株材料来源背景不同,未表现出明确的地域性规律。
关键词:  新麦草  SSR分子标记  遗传多样性  群体结构  主成分分析  NJ聚类
DOI:10.13430/j.cnki.jpgr.20180612002
投稿时间:2018-06-12修订日期:2018-11-15
基金项目:国家自然科学基金项目(31460627);内蒙古农业大学科技成果转化专项(YZGC2017012);
Genetic Diversity and Structure Analysis in Psathyrostachys Population Using SSR Markers
ZHANG Chen1, YUN Lan1,2, LI Zhen1, WANG Jun1, GUO Hong-yu1, SHENG Yu1, SHI Zi-ying1, XU Xue-bao1
(1College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010018;2Forage Germplasm improving and Breeding Laboratory and Key Lab of Grassland Resources Ministry of Education P.R. China, Hohhot 010018)
Abstract:
Genetic diversity and population structure analysis of 171 wild and cultivated Psathyrostachys individual germplasms from different countries and regions were conducted by 38 pairs of SSR primers. The result by using the effective primers revealed a total of 308 loci among Psathyrostachys individuals, with 275 polymorphic loci and a polymorphism percentage of 89.29%. The genetic similarity coefficient of the tested materials ranged from 0.537 to 0.899 with an average of 0.742. These accessions were divided into three groups by population structure analysis, principal component analysis and NJ clustering. The genetic similarity coefficient analysis showed that the distribution of Psathyrostachys individuals was more concentrated. The classification using three methods generally revealed three different groups of Psathyrostachys materials, despite that there were slightly different in the divisions of mixed groups. Since these Psathyrostachys individuals in each group showed different on genetic background, no clear correlation between geographic distribution and genetic diversity was observed.
Key words:  Psathyrostachys juncea  SSR  genetic diversity  population structure  principal component analysis  NJ clustering

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